Difference between revisions of "PGP888"
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The vector was constructed in the [[Stülke]] lab and it is suitable for the xylose-inducible expression of proteins in ''B. subtilis''. The plasmid confers resistance to ampicillin and kanamycin in ''E. coli'' and ''B. subtilis'', respectively. To transform ''B. subtilis'', the plasmid has to be linearized with ScaI or NotI | The vector was constructed in the [[Stülke]] lab and it is suitable for the xylose-inducible expression of proteins in ''B. subtilis''. The plasmid confers resistance to ampicillin and kanamycin in ''E. coli'' and ''B. subtilis'', respectively. To transform ''B. subtilis'', the plasmid has to be linearized with ScaI or NotI | ||
Revision as of 11:44, 26 August 2011
The vector was constructed in the Stülke lab and it is suitable for the xylose-inducible expression of proteins in B. subtilis. The plasmid confers resistance to ampicillin and kanamycin in E. coli and B. subtilis, respectively. To transform B. subtilis, the plasmid has to be linearized with ScaI or NotI
Hints for usage
- An additional base has to be inserted in the forward primer to restore the frame.
- Don't forget a stop codon in the reverse primer.
The reference
Christine Diethmaier, Nico Pietack, Katrin Gunka, Christoph Wrede, Martin Lehnik-Habrink, Christina Herzberg, Sebastian Hübner, Jörg Stülke
A novel factor controlling bistability in Bacillus subtilis: the YmdB protein affects flagellin expression and biofilm formation.
J Bacteriol: 2011, 193(21);5997-6007
[PubMed:21856853]
[WorldCat.org]
[DOI]
(I p)
