Difference between revisions of "YwlE"
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=Biological materials = | =Biological materials = | ||
| − | * '''Mutant:''' | + | * '''Mutant:''' ''ywlE::aphA3'' availbale from the Gerth lab |
| + | ''ywlE::tet'' availbale from the Gerth lab | ||
| + | ''ywlE::spec'' availbale from the Gerth lab | ||
| − | * '''Expression vector:''' | + | * '''Expression vector:''' for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab |
* '''lacZ fusion:''' | * '''lacZ fusion:''' | ||
| Line 119: | Line 121: | ||
* '''two-hybrid system:''' | * '''two-hybrid system:''' | ||
| − | * '''Antibody:''' | + | * '''Antibody:''' available in Gerth lab |
=Labs working on this gene/protein= | =Labs working on this gene/protein= | ||
Revision as of 20:22, 6 September 2011
- Description: protein tyrosine phosphatase, protein arginine phosphatase
| Gene name | ywlE |
| Synonyms | ipc-31d |
| Essential | no |
| Product | protein tyrosine phosphatase, protein arginine phosphatase |
| Function | dephosphorylation of McsB |
| MW, pI | 16 kDa, 6.681 |
| Gene length, protein length | 450 bp, 150 aa |
| Immediate neighbours | ywlF, ywlD |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU36930
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Protein tyrosine phosphate + H2O = protein tyrosine + phosphate (according to Swiss-Prot)
- Protein family: low molecular weight phosphotyrosine protein phosphatase family (according to Swiss-Prot)
- Paralogous protein(s): YfkJ
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: weak oligomerization, results in inactivation of YwlE PubMed
Database entries
- Structure: 1ZGG
- UniProt: P39155
- KEGG entry: [2]
- E.C. number: 3.1.3.48
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: ywlE::aphA3 availbale from the Gerth lab
ywlE::tet availbale from the Gerth lab ywlE::spec availbale from the Gerth lab
- Expression vector: for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody: available in Gerth lab
Labs working on this gene/protein
Your additional remarks
References
