ProJ

• Description: glutamate 5-kinase
  
  

Gene name	proJ
Synonyms	yohA
Essential	no
Product	glutamate 5-kinase
Function	osmoadaptive de novo production of proline
Metabolic function and regulation of this protein in SubtiPathways: Pro, Stress
MW, pI	40 kDa, 5.296
Gene length, protein length	1113 bp, 371 aa
Immediate neighbours	gltC, proH
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, coping with hyper-osmotic stress

The gene

Basic information

• Locus tag: BSU18470

Expression

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + L-glutamate = ADP + L-glutamate 5-phosphate (according to Swiss-Prot)

• Protein family:

• Paralogous protein(s): ProB

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O07509

• KEGG entry: [2]

• E.C. number: 2.7.2.11

Expression and regulation

• Operon: proH-proJ PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed under conditions of osmotic stress PubMed
  • induced by cold stress PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP817 (spc) available in the Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Tamara Hoffmann, Erhard Bremer
Protection of Bacillus subtilis against cold stress via compatible-solute acquisition.
J Bacteriol: 2011, 193(7);1552-62
[PubMed:21296969] [WorldCat.org] [DOI] (I p)