Difference between revisions of "SepF"

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(Extended information on the protein)
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* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
  
* '''Interactions:''' [[SepF]]-[[FtsZ]] {{PubMed|16420366}}
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* '''[[SubtInteract|Interactions]]:'''  
 +
** [[SepF]]-[[FtsZ]] {{PubMed|16420366}}
 
   
 
   
* '''Localization:''' septum {{PubMed|16420366}}
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* '''[[Localization]]:''' septum {{PubMed|16420366}}
  
 
=== Database entries ===
 
=== Database entries ===

Revision as of 17:01, 10 August 2011

  • Description: part of the divisome

Gene name sepF
Synonyms ylmF
Essential no
Product FtsZ-interacting protein
Function proper execution of septum synthesis
Interactions involving this protein in SubtInteract: SepF
MW, pI 17 kDa, 4.863
Gene length, protein length 447 bp, 149 aa
Immediate neighbours ylmE, ylmG
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YlmF context.gif
This image was kindly provided by SubtiList





Categories containing this gene/protein

cell division

This gene is a member of the following regulons

Spo0A regulon

The gene

Basic information

  • Locus tag: BSU15390

Phenotypes of a mutant

Less efficient cell division results in longer cells. Electron microscopy reveals strongly distorted division septa.

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • SepF assembles into very large (∼50 nm diameter) rings. These rings are able to bundle FtsZ protofilaments into strikingly long and regular tubular structures reminiscent of eukaryotic microtubules PubMed
  • Protein family: sepF family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed under conditions that trigger sporulation (Spo0A) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Leendert Hamoen, CBCB, Newcastle University, UK

Shu Ishikawa, Nara Institute of Science and Technology, Nara, Japan

Your additional remarks

SepF mutation is synthetic lethal in combination with an ezrA mutation or an ftsA mutation.

References

Reviews

David W Adams, Jeff Errington
Bacterial cell division: assembly, maintenance and disassembly of the Z ring.
Nat Rev Microbiol: 2009, 7(9);642-53
[PubMed:19680248] [WorldCat.org] [DOI] (I p)


Original Publications

Additional publications: PubMed

Shu Ishikawa, Yoshikazu Kawai, Konosuke Hiramatsu, Masayoshi Kuwano, Naotake Ogasawara
A new FtsZ-interacting protein, YlmF, complements the activity of FtsA during progression of cell division in Bacillus subtilis.
Mol Microbiol: 2006, 60(6);1364-80
[PubMed:16796675] [WorldCat.org] [DOI] (P p)

Leendert W Hamoen, Jean-Christophe Meile, Wouter de Jong, Philippe Noirot, Jeff Errington
SepF, a novel FtsZ-interacting protein required for a late step in cell division.
Mol Microbiol: 2006, 59(3);989-99
[PubMed:16420366] [WorldCat.org] [DOI] (P p)

Virginie Molle, Masaya Fujita, Shane T Jensen, Patrick Eichenberger, José E González-Pastor, Jun S Liu, Richard Losick
The Spo0A regulon of Bacillus subtilis.
Mol Microbiol: 2003, 50(5);1683-701
[PubMed:14651647] [WorldCat.org] [DOI] (P p)