NagA

• Description: N-acetylglucosamine-6-phosphate deacetylase
  
  

Gene name	nagA
Synonyms
Essential	no
Product	N-acetylglucosamine-6-phosphate deacetylase
Function	N-acetylglucosamine utilization
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism, Murein recycling
MW, pI	42 kDa, 5.276
Gene length, protein length	1188 bp, 396 aa
Immediate neighbours	hprK, nagB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

cell wall degradation/ turnover, utilization of specific carbon sources

This gene is a member of the following regulons

CcpA regulon, NagR regulon

The gene

Basic information

• Locus tag: BSU35010

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: N-acetyl-D-glucosamine 6-phosphate + H₂O = D-glucosamine 6-phosphate + acetate (according to Swiss-Prot)

• Protein family: nagA family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information: K(M): 1.4 mM PubMed

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2VHL PubMed

• UniProt: O34450

• KEGG entry: [2]

• E.C. number: 3.5.1.25

Expression and regulation

• Operon: nagA-nagB-nagR PubMed

• Sigma factor:

• Regulation:
  • induced in the presence of N-acetylglucosamine (NagR) PubMed
  • repressed by glucose (2.9-fold) (CcpA) PubMed

• Regulatory mechanism:
  • NagR: transcription repression PubMed
  • CcpA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed