Difference between revisions of "NagA"
| Line 39: | Line 39: | ||
= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = | ||
{{SubtiWiki regulon|[[CcpA regulon]]}}, | {{SubtiWiki regulon|[[CcpA regulon]]}}, | ||
| − | {{SubtiWiki regulon|[[ | + | {{SubtiWiki regulon|[[NagR regulon]]}} |
=The gene= | =The gene= | ||
| Line 56: | Line 56: | ||
=== Additional information=== | === Additional information=== | ||
| − | |||
| − | |||
| − | |||
=The protein= | =The protein= | ||
| Line 99: | Line 96: | ||
=Expression and regulation= | =Expression and regulation= | ||
| − | + | * '''Operon:''' ''[[nagA]]-[[nagB]]-[[nagR]]'' {{PubMed|21602348}} | |
| − | * '''Operon:''' | ||
* '''[[Sigma factor]]:''' | * '''[[Sigma factor]]:''' | ||
* '''Regulation:''' | * '''Regulation:''' | ||
| − | ** induced | + | ** induced in the presence of N-acetylglucosamine ([[NagR]]) {{PubMed|21602348,14343123}} |
** repressed by glucose (2.9-fold) ([[CcpA]]) [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed] | ** repressed by glucose (2.9-fold) ([[CcpA]]) [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed] | ||
| − | * '''Regulatory mechanism:''' [[CcpA]]: transcription repression [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed] | + | * '''Regulatory mechanism:''' |
| + | ** [[NagR]]: transcription repression {{PubMed|21602348}} | ||
| + | ** [[CcpA]]: transcription repression [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed] | ||
* '''Additional information:''' | * '''Additional information:''' | ||
| Line 131: | Line 129: | ||
=References= | =References= | ||
| − | + | '''Additional publications:''' {{PubMed|21602348}} | |
<pubmed>10627040, 14343123,12850135 6174502, 14557261 </pubmed> | <pubmed>10627040, 14343123,12850135 6174502, 14557261 </pubmed> | ||
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 20:20, 24 May 2011
- Description: N-acetylglucosamine-6-phosphate deacetylase
| Gene name | nagA |
| Synonyms | |
| Essential | no |
| Product | N-acetylglucosamine-6-phosphate deacetylase |
| Function | N-acetylglucosamine utilization |
| Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism, Murein recycling | |
| MW, pI | 42 kDa, 5.276 |
| Gene length, protein length | 1188 bp, 396 aa |
| Immediate neighbours | hprK, nagB |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
Categories containing this gene/protein
cell wall degradation/ turnover, utilization of specific carbon sources
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU35010
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: N-acetyl-D-glucosamine 6-phosphate + H2O = D-glucosamine 6-phosphate + acetate (according to Swiss-Prot)
- Protein family: nagA family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information: K(M): 1.4 mM PubMed
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- UniProt: O34450
- KEGG entry: [2]
- E.C. number: 3.5.1.25
Additional information
Expression and regulation
- Regulation:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Additional publications: PubMed
Florence Vincent, David Yates, Elspeth Garman, Gideon J Davies, James A Brannigan
The three-dimensional structure of the N-acetylglucosamine-6-phosphate deacetylase, NagA, from Bacillus subtilis: a member of the urease superfamily.
J Biol Chem: 2004, 279(4);2809-16
[PubMed:14557261]
[WorldCat.org]
[DOI]
(P p)
Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135]
[WorldCat.org]
[DOI]
(P p)
Jonathan Reizer, Steffi Bachem, Aiala Reizer, Maryvonne Arnaud, Milton H Saier, Jörg Stülke
Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis.
Microbiology (Reading): 1999, 145 ( Pt 12);3419-3429
[PubMed:10627040]
[WorldCat.org]
[DOI]
(P p)
H L Mobley, R J Doyle, U N Streips, S O Langemeier
Transport and incorporation of N-acetyl-D-glucosamine in Bacillus subtilis.
J Bacteriol: 1982, 150(1);8-15
[PubMed:6174502]
[WorldCat.org]
[DOI]
(P p)
C J BATES, C A PASTERNAK
FURTHER STUDIES ON THE REGULATION OF AMINO SUGAR METABOLISM IN BACILLUS SUBTILIS.
Biochem J: 1965, 96(1);147-54
[PubMed:14343123]
[WorldCat.org]
[DOI]
(P p)
