Difference between revisions of "UvrA"
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=Biological materials = | =Biological materials = | ||
| − | * '''mutant:''' GP1175 (''uvrAB''::''ermC'') (available in the [[Stülke]] lab) | + | * '''mutant:''' GP1175 (''del uvrAB''::''ermC'') (available in the [[Stülke]] lab) |
* '''Expression vector:''' | * '''Expression vector:''' | ||
Revision as of 16:09, 7 February 2011
- Description: excinuclease ABC (subunit A)
| Gene name | uvrA |
| Synonyms | |
| Essential | no |
| Product | excinuclease ABC (subunit A) |
| Function | DNA repair after UV damage |
| MW, pI | 105 kDa, 5.843 |
| Gene length, protein length | 2871 bp, 957 aa |
| Immediate neighbours | yvzB, uvrB |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU35160
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: UvrA subfamily (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure: 2R6F (Geobacillus stearothermophilus)
- UniProt: O34863
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Additional information:
Biological materials
- mutant: GP1175 (del uvrAB::ermC) (available in the Stülke lab)
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
James J Truglio, Deborah L Croteau, Bennett Van Houten, Caroline Kisker
Prokaryotic nucleotide excision repair: the UvrABC system.
Chem Rev: 2006, 106(2);233-52
[PubMed:16464004]
[WorldCat.org]
[DOI]
(P p)
Bennett Van Houten, Deborah L Croteau, Matthew J DellaVecchia, Hong Wang, Caroline Kisker
'Close-fitting sleeves': DNA damage recognition by the UvrABC nuclease system.
Mutat Res: 2005, 577(1-2);92-117
[PubMed:15927210]
[WorldCat.org]
[DOI]
(P p)
A Sancar
Mechanisms of DNA excision repair.
Science: 1994, 266(5193);1954-6
[PubMed:7801120]
[WorldCat.org]
[DOI]
(P p)
Original Publications
