PucK

• Description: uric acid permease
  
  

Gene name	pucK
Synonyms	yunK
Essential	no
Product	uric acid permease
Function	purine utilization
Metabolic function and regulation of this protein in SubtiPathways: Purine catabolism, Nucleotides (regulation)
MW, pI	44 kDa, 8.348
Gene length, protein length	1290 bp, 430 aa
Immediate neighbours	pucJ, pucL
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

transporters/ other, utilization of nucleotides, membrane proteins

This gene is a member of the following regulons

PucR regulon, TnrA regulon

The gene

Basic information

• Locus tag: BSU32440

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: xanthine/uracil permease family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O32140

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:
  • pucR-pucJ-pucK-pucL-pucM PubMed
  • pucJ-pucK-pucL-pucM PubMed

• Sigma factor:
  • pucJ: SigA PubMed

• Regulation:
  • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
  • induced in the presence of purine nucleotides (inducer: allantoin) (PucR) PubMed

• Regulatory mechanism:
  • TnrA: transcription activation PubMed
  • PucR: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: