Difference between revisions of "BglS"

From SubtiWiki
Jump to: navigation, search
(Expression and regulation)
(Expression and regulation)
Line 88: Line 88:
 
=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:''' ''[[licT]]-[[bglS]]''
+
* '''Operon:''' ''[[licT]]-[[bglS]]'', ''[[bglS]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/8606172 PubMed]
  
 
* '''Sigma factor:''' [[SigA]]
 
* '''Sigma factor:''' [[SigA]]
  
* '''Regulation:''' expressed in the stationary phase (temporal activation), carbon catabolite repression ([[CcpA]]), induction by ß-glucosides ([[LicT]])  
+
* '''Regulation:''' expressed in the stationary phase (temporal activation), carbon catabolite repression ([[CcpA]]), weak induction by ß-glucosides ([[LicT]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/8606172 PubMed]
* '''Regulatory mechanism:''' [[CcpA]]: transcription repression, [[LicT]]: binding to an RNA switch results in transcriptional antitermination
+
* '''Regulatory mechanism:''' [[CcpA]]: transcription repression, [[LicT]]: binding to an RNA switch results in transcriptional antitermination [http://www.ncbi.nlm.nih.gov/sites/entrez/8606172 PubMed]
  
 
* '''Additional information:'''
 
* '''Additional information:'''

Revision as of 15:46, 8 February 2009

  • Description: write here

Gene name bglS
Synonyms bgl, licS
Essential
Product endo-beta-1,3-1,4 glucanase (EC 3.2.1.73)
Function
MW, pI 27 kDa, 6.482
Gene length, protein length 726 bp, 242 aa
Immediate neighbours
Gene sequence (+200bp) Protein sequence
Genetic context
BglS context.gif



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry:
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: expressed in the stationary phase (temporal activation), carbon catabolite repression (CcpA), weak induction by ß-glucosides (LicT) PubMed
  • Regulatory mechanism: CcpA: transcription repression, LicT: binding to an RNA switch results in transcriptional antitermination PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Murphy ND, McConnell DJ, Cantwell BA. 1984. The DNA sequence of the gene and genetic control sites for the excreted Bacillus subtilis enzyme β-glucanase. Nucl. Acids Res. 12:5355-5367. PubMed
  2. Stülke, J., Hanschke, R. & Hecker, M. (1993) Temporal activation of ß-glucanase synthesis in Bacillus subtilis is mediated by the GTP pool. J. Gen. Microbiol. 139: 2041-2045. PubMed
  3. Krüger, S., Stülke, J. & Hecker, M. (1993) Carbon catabolite repression of ß-glucanase synthesis in Bacillus subtilis. J. Gen. Microbiol. 139: 2047-2054. PubMed
  4. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed