Difference between revisions of "LcfB"

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= This gene is a member of the following [[regulons]] =
 
= This gene is a member of the following [[regulons]] =
{{SubtiWiki regulon|[[FadR regulon]]}}
+
{{SubtiWiki regulon|[[CcpA regulon]]}}, {{SubtiWiki regulon|[[FadR regulon]]}}
  
 
=The gene=
 
=The gene=
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* '''Regulation:'''  
 
* '''Regulation:'''  
 
** induced in the presence of long-chain fatty acids ([[FadR]]) {{PubMed|17189250}}
 
** induced in the presence of long-chain fatty acids ([[FadR]]) {{PubMed|17189250}}
 +
** subject to carbon catabolite repression ([[CcpA]]-[[PtsH|HPr(Ser-P)]] {{PubMed|21398533}}
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
 
** [[FadR]]: transcription repression {{PubMed|17189250}}
 
** [[FadR]]: transcription repression {{PubMed|17189250}}
 +
** [[CcpA]]-[[PtsH|HPr(Ser-P)]]: transcription repression {{PubMed|21398533}}
  
 
* '''Additional information:'''
 
* '''Additional information:'''
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=References=
 
=References=
 
+
'''Additional publications:''' {{PubMed|21398533}}
 
<pubmed>17189250,17919287 20797616</pubmed>
 
<pubmed>17189250,17919287 20797616</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 12:57, 19 March 2011

  • Description: long-chain fatty-acid-CoA ligase, involved in surfactin production

Gene name lcfB
Synonyms yhfL
Essential no
Product long-chain fatty-acid-CoA ligase
Function fatty acid degradation
Metabolic function and regulation of this protein in SubtiPathways:
Fatty acid degradation
MW, pI 56 kDa, 5.301
Gene length, protein length 1539 bp, 513 aa
Immediate neighbours yhfK, yhfM
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YhfL context.gif
This image was kindly provided by SubtiList







Categories containing this gene/protein

utilization of lipids

This gene is a member of the following regulons

CcpA regulon, FadR regulon

The gene

Basic information

  • Locus tag: BSU10270

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA (according to Swiss-Prot), activates 3-hydroxy fatty acids for surfactin biosynthesis PubMed
  • Protein family: ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed