LytF

• Description: gamma-D-glutamate-meso-diaminopimelate muropeptidase (major autolysin)
  
  

Gene name	lytF
Synonyms	cwlE, yhdD
Essential	no
Product	gamma-D-glutamate-meso-diaminopimelate muropeptidase (major autolysin)
Function	cell separation
Interactions involving this protein in SubtInteract: LytF
MW, pI	51 kDa, 10.41
Gene length, protein length	1464 bp, 488 aa
Immediate neighbours	yhdC, nsrR
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

cell wall degradation/ turnover

This gene is a member of the following regulons

SigD regulon, SlrR regulon

The gene

Basic information

• Locus tag: BSU09370

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: nlpC/p60 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: IseA-LytF (catalytic domain) PubMed

• Localization: secreted (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O07532

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: lytF PubMed

• Sigma factor: SigD PubMed

• Regulation:

• Regulatory mechanism:
  • SlrR (in complex with SinR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Yunrong Chai, Thomas Norman, Roberto Kolter, Richard Losick
An epigenetic switch governing daughter cell separation in Bacillus subtilis.
Genes Dev: 2010, 24(8);754-65
[PubMed:20351052] [WorldCat.org] [DOI] (I p)

Rui Chen, Sarah B Guttenplan, Kris M Blair, Daniel B Kearns
Role of the sigmaD-dependent autolysins in Bacillus subtilis population heterogeneity.
J Bacteriol: 2009, 191(18);5775-84
[PubMed:19542270] [WorldCat.org] [DOI] (I p)

Hiroki Yamamoto, Yukiko Miyake, Miharu Hisaoka, Shin-Ichirou Kurosawa, Junichi Sekiguchi
The major and minor wall teichoic acids prevent the sidewall localization of vegetative DL-endopeptidase LytF in Bacillus subtilis.
Mol Microbiol: 2008, 70(2);297-310
[PubMed:18761696] [WorldCat.org] [DOI] (I p)

Hiroki Yamamoto, Shin-ichirou Kurosawa, Junichi Sekiguchi
Localization of the vegetative cell wall hydrolases LytC, LytE, and LytF on the Bacillus subtilis cell surface and stability of these enzymes to cell wall-bound or extracellular proteases.
J Bacteriol: 2003, 185(22);6666-77
[PubMed:14594841] [WorldCat.org] [DOI] (P p)

R Ohnishi, S Ishikawa, J Sekiguchi
Peptidoglycan hydrolase LytF plays a role in cell separation with CwlF during vegetative growth of Bacillus subtilis.
J Bacteriol: 1999, 181(10);3178-84
[PubMed:10322020] [WorldCat.org] [DOI] (P p)

Philippe Margot, Marco Pagni, Dimitri Karamata
Bacillus subtilis 168 gene lytF encodes a gamma-D-glutamate-meso-diaminopimelate muropeptidase expressed by the alternative vegetative sigma factor, sigmaD.
Microbiology (Reading): 1999, 145 ( Pt 1);57-65
[PubMed:10206711] [WorldCat.org] [DOI] (P p)