Difference between revisions of "GuaA"
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=== Additional information=== | === Additional information=== | ||
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=The protein= | =The protein= | ||
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* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
| + | ** the enzyme is inhibited by guanosine tetraphosphate ([[stringent response]]) {{PubMed|409404}} | ||
* '''Interactions:''' | * '''Interactions:''' | ||
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=References= | =References= | ||
| − | <pubmed>1722815,1312531, </pubmed> | + | <pubmed>1722815,1312531, 409404 </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 08:50, 14 February 2011
- Description: GMP synthase (glutamine-hydrolysing)
| Gene name | guaA |
| Synonyms | guaB |
| Essential | no |
| Product | GMP synthetase (glutamine-hydrolysing) |
| Function | biosynthesis of GMP |
| Metabolic function and regulation of this protein in SubtiPathways: Purine synthesis, Nucleotides (regulation) | |
| MW, pI | 57 kDa, 4.747 |
| Gene length, protein length | 1539 bp, 513 aa |
| Immediate neighbours | yebA, pbuG |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
Categories containing this gene/protein
biosynthesis/ acquisition of nucleotides
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU06360
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate (according to Swiss-Prot)
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- the enzyme is inhibited by guanosine tetraphosphate (stringent response) PubMed
- Interactions:
- Localization:
Database entries
- Structure: 2YWB (from Thermus thermophilus hb8, 45% identity, 57% similarity)
- UniProt: P29727
- KEGG entry: [3]
- E.C. number: 6.3.5.2
Additional information
Expression and regulation
- Operon: guaA PubMed
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
P Mäntsälä, H Zalkin
Cloning and sequence of Bacillus subtilis purA and guaA, involved in the conversion of IMP to AMP and GMP.
J Bacteriol: 1992, 174(6);1883-90
[PubMed:1312531]
[WorldCat.org]
[DOI]
(P p)
H H Saxild, P Nygaard
Regulation of levels of purine biosynthetic enzymes in Bacillus subtilis: effects of changing purine nucleotide pools.
J Gen Microbiol: 1991, 137(10);2387-94
[PubMed:1722815]
[WorldCat.org]
[DOI]
(P p)
T Mitani, J E Heinze, E Freese
Induction of sporulation in Bacillus subtilis by decoyinine or hadacidin.
Biochem Biophys Res Commun: 1977, 77(3);1118-25
[PubMed:409404]
[WorldCat.org]
[DOI]
(P p)
