Difference between revisions of "RplF"

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|style="background:#ABCDEF;" align="center"|'''Function''' || translation  
 
|style="background:#ABCDEF;" align="center"|'''Function''' || translation  
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/RplF RplF]
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 19 kDa, 9.916   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 19 kDa, 9.916   
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* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
  
* '''Interactions:''' [[YsxC]]-[[RplF]] [http://www.ncbi.nlm.nih.gov/sites/entrez/17981968 PubMed]
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* '''[[SubtInteract|Interactions]]:'''
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** [[YsxC]]-[[RplF]] [http://www.ncbi.nlm.nih.gov/sites/entrez/17981968 PubMed]
  
* '''Localization:'''
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* '''[[Localization]]:'''
  
 
=== Database entries ===
 
=== Database entries ===

Revision as of 10:05, 23 July 2011

Gene name rplF
Synonyms
Essential yes PubMed
Product ribosomal protein L6 (BL8)
Function translation
Interactions involving this protein in SubtInteract: RplF
MW, pI 19 kDa, 9.916
Gene length, protein length 537 bp, 179 aa
Immediate neighbours rpsH, rplR
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
RplF context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

translation, essential genes, universally conserved proteins

This gene is a member of the following regulons

stringent response

The gene

Basic information

  • Locus tag: BSU01310

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1RL6 (Geobacillus stearothermophilus), 2R6A (Geobacillus stearothermophilus), 2R6C, 2R6D, 2R6E
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
    • SigA two promoters, about 140bp and 200bp upstream of the start codon PubMed
  • Regulation:
    • RelA dependent downregulation (Class I) during stringent response PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Matthew A Lauber, William E Running, James P Reilly
B. subtilis ribosomal proteins: structural homology and post-translational modifications.
J Proteome Res: 2009, 8(9);4193-206
[PubMed:19653700] [WorldCat.org] [DOI] (P p)

Catherine Wicker-Planquart, Anne-Emmanuelle Foucher, Mathilde Louwagie, Robert A Britton, Jean-Michel Jault
Interactions of an essential Bacillus subtilis GTPase, YsxC, with ribosomes.
J Bacteriol: 2008, 190(2);681-90
[PubMed:17981968] [WorldCat.org] [DOI] (I p)

Christine Eymann, Georg Homuth, Christian Scharf, Michael Hecker
Bacillus subtilis functional genomics: global characterization of the stringent response by proteome and transcriptome analysis.
J Bacteriol: 2002, 184(9);2500-20
[PubMed:11948165] [WorldCat.org] [DOI] (P p)

X Li, L Lindahl, Y Sha, J M Zengel
Analysis of the Bacillus subtilis S10 ribosomal protein gene cluster identifies two promoters that may be responsible for transcription of the entire 15-kilobase S10-spc-alpha cluster.
J Bacteriol: 1997, 179(22);7046-54
[PubMed:9371452] [WorldCat.org] [DOI] (P p)

J W Suh, S A Boylan, S H Oh, C W Price
Genetic and transcriptional organization of the Bacillus subtilis spc-alpha region.
Gene: 1996, 169(1);17-23
[PubMed:8635744] [WorldCat.org] [DOI] (P p)