Difference between revisions of "YhfR"
| Line 30: | Line 30: | ||
<br/><br/> | <br/><br/> | ||
| + | |||
| + | = [[Categories]] containing this gene/protein = | ||
| + | {{SubtiWiki category|[[biosynthesis of lipids]]}} | ||
| + | |||
| + | = This gene is a member of the following [[regulons]] = | ||
| + | |||
=The gene= | =The gene= | ||
| Line 51: | Line 57: | ||
| − | + | ||
| − | |||
=The protein= | =The protein= | ||
Revision as of 18:13, 8 December 2010
- Description: phosphatase involved in isopentenol (isoprenoid) biosynthesis
| Gene name | yhfR |
| Synonyms | |
| Essential | no |
| Product | unknown |
| Function | isoprenoid biosynthesis |
| MW, pI | 21 kDa, 5.164 |
| Gene length, protein length | 579 bp, 193 aa |
| Immediate neighbours | yhfQ, yhfS |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU10340
Phenotypes of a mutant
no detectable phenotype PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: phosphatase involved in isopentenol (isoprenoid) biosynthesis
- Protein family: GpmB subfamily (according to Swiss-Prot) similar to 2,3-diphosphoglycerate-dependent phosphoglycerate mutases PubMed
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- UniProt: O07617
- KEGG entry: [2]
- E.C. number:
Additional information
The gene is annotated in KEGG as an ortholog of phosphoglycerate mutase (PGM) EC 5.4.2.1. In MetaCyc the protein is marked as “similar to phosphoglycerate mutase”. No EC annotation is available in Swiss-Prot. Pearson et al. (PubMed) demonstrated that yhfR is non-essential for growth, sporulation, and spore germination. They also purified the gene, expressed it in B. subtilis but were not able to detect PGM activity in B. subtilis. PubMed
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information: weakly expressed PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
