Difference between revisions of "MetN"

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= [[Categories]] containing this gene/protein =
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{{SubtiWiki category|[[ABC transporters]]}},
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{{SubtiWiki category|[[biosynthesis/ acquisition of amino acids]]}},
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{{SubtiWiki category|[[membrane proteins]]}}
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= This gene is a member of the following [[regulons]] =
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{{SubtiWiki regulon|[[CodY regulon]]}},
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{{SubtiWiki regulon|[[S-box]]}}
  
 
=The gene=
 
=The gene=
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= Categories containing this gene/protein =
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{{SubtiWiki category|[[ABC transporters]]}},
 
{{SubtiWiki category|[[biosynthesis/ acquisition of amino acids]]}},
 
{{SubtiWiki category|[[membrane proteins]]}}
 
 
=The protein=
 
=The protein=
  

Revision as of 23:10, 8 December 2010

Gene name metN
Synonyms yusC
Essential no
Product methionine ABC transporter (ATP-binding protein)
Function methionine uptake
Metabolic function and regulation of this protein in SubtiPathways:
Cys, Met & Sulfate assimilation
MW, pI 37 kDa, 7.303
Gene length, protein length 1023 bp, 341 aa
Immediate neighbours metP, yusD
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YusC context.gif
This image was kindly provided by SubtiList







Categories containing this gene/protein

ABC transporters, biosynthesis/ acquisition of amino acids, membrane proteins

This gene is a member of the following regulons

CodY regulon, S-box

The gene

Basic information

  • Locus tag: BSU32750

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization: associated to the cell membrane, via MetP PubMed

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
    • activated during growth in the presence of branched chain amino acids (CodY) PubMed
    • induced by methionine starvation (S-box) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Marie-Françoise Hullo, Sandrine Auger, Elie Dassa, Antoine Danchin, Isabelle Martin-Verstraete
The metNPQ operon of Bacillus subtilis encodes an ABC permease transporting methionine sulfoxide, D- and L-methionine.
Res Microbiol: 2004, 155(2);80-6
[PubMed:14990259] [WorldCat.org] [DOI] (P p)

Virginie Molle, Yoshiko Nakaura, Robert P Shivers, Hirotake Yamaguchi, Richard Losick, Yasutaro Fujita, Abraham L Sonenshein
Additional targets of the Bacillus subtilis global regulator CodY identified by chromatin immunoprecipitation and genome-wide transcript analysis.
J Bacteriol: 2003, 185(6);1911-22
[PubMed:12618455] [WorldCat.org] [DOI] (P p)

F J Grundy, T M Henkin
The S box regulon: a new global transcription termination control system for methionine and cysteine biosynthesis genes in gram-positive bacteria.
Mol Microbiol: 1998, 30(4);737-49
[PubMed:10094622] [WorldCat.org] [DOI] (P p)

Y Quentin, G Fichant, F Denizot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J Mol Biol: 1999, 287(3);467-84
[PubMed:10092453] [WorldCat.org] [DOI] (P p)