KsgA

• Description: rRNA adenine dimethyltransferase
  
  

Gene name	ksgA
Synonyms
Essential	no
Product	rRNA adenine dimethyltransferase
Function	rRNA maturation
MW, pI	32 kDa, 5.712
Gene length, protein length	876 bp, 292 aa
Immediate neighbours	rnmV, yabG
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU00420

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: methylation of A1518 and A1519 in 16S rRNA

• Protein family: KsgA subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P37468

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: rnmV-ksgA PubMed

• Sigma factor: SigF PubMed

• Regulation: expressed early during sporulation in the forespore (SigF) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Kozo Ochi, Ji-Yun Kim, Yukinori Tanaka, Guojun Wang, Kenta Masuda, Hideaki Nanamiya, Susumu Okamoto, Shinji Tokuyama, Yoshikazu Adachi, Fujio Kawamura
Inactivation of KsgA, a 16S rRNA methyltransferase, causes vigorous emergence of mutants with high-level kasugamycin resistance.
Antimicrob Agents Chemother: 2009, 53(1);193-201
[PubMed:19001112] [WorldCat.org] [DOI] (I p)

Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)

C Condon, D Brechemier-Baey, B Beltchev, M Grunberg-Manago, H Putzer
Identification of the gene encoding the 5S ribosomal RNA maturase in Bacillus subtilis: mature 5S rRNA is dispensable for ribosome function.
RNA: 2001, 7(2);242-53
[PubMed:11233981] [WorldCat.org] [DOI] (P p)