Difference between revisions of "KsgA"

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|style="background:#ABCDEF;" align="center"| '''Product''' || rRNA adenine dimethyltransferase
 
|style="background:#ABCDEF;" align="center"| '''Product''' || rRNA adenine dimethyltransferase
 
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|style="background:#ABCDEF;" align="center"|'''Function''' || resistance to kasugamycin
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|style="background:#ABCDEF;" align="center"|'''Function''' || rRNA maturation
 
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|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 32 kDa, 5.712   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 32 kDa, 5.712   

Revision as of 15:15, 18 June 2010

  • Description: rRNA adenine dimethyltransferase

Gene name ksgA
Synonyms
Essential no
Product rRNA adenine dimethyltransferase
Function rRNA maturation
MW, pI 32 kDa, 5.712
Gene length, protein length 876 bp, 292 aa
Immediate neighbours rnmV, yabG
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
KsgA context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU00420

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: methylation of A1518 and A1519 in 16S rRNA
  • Protein family: KsgA subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: expressed early during sporulation in the forespore (SigF) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Kozo Ochi, Ji-Yun Kim, Yukinori Tanaka, Guojun Wang, Kenta Masuda, Hideaki Nanamiya, Susumu Okamoto, Shinji Tokuyama, Yoshikazu Adachi, Fujio Kawamura
Inactivation of KsgA, a 16S rRNA methyltransferase, causes vigorous emergence of mutants with high-level kasugamycin resistance.
Antimicrob Agents Chemother: 2009, 53(1);193-201
[PubMed:19001112] [WorldCat.org] [DOI] (I p)

Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)

C Condon, D Brechemier-Baey, B Beltchev, M Grunberg-Manago, H Putzer
Identification of the gene encoding the 5S ribosomal RNA maturase in Bacillus subtilis: mature 5S rRNA is dispensable for ribosome function.
RNA: 2001, 7(2);242-53
[PubMed:11233981] [WorldCat.org] [DOI] (P p)