PhoB

• Description: alkaline phosphatase III
  
  

Gene name	phoB
Synonyms	phoAIII
Essential	no
Product	alkaline phosphatase III
Function	aquisition of phosphate upon phosphoate starvation
Metabolic function and regulation of this protein in SubtiPathways: Folate
MW, pI	50 kDa, 5.895
Gene length, protein length	1386 bp, 462 aa
Immediate neighbours	ydhF, ydhG
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU05740

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

phosphate metabolism, sporulation proteins

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: A phosphate monoester + H₂O = an alcohol + phosphate (according to Swiss-Prot)

• Protein family: alkaline phosphatase family (according to Swiss-Prot)

• Paralogous protein(s): PhoA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylated on ser/ thr/ tyr PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: extracellular (signal peptide) PubMed

Database entries

• Structure:

• UniProt: P19405

• KEGG entry: [3]

• E.C. number: 3.1.3.1

Expression and regulation

• Operon: phoB-ydhF PubMed

• Sigma factor: SigA, SigE PubMed

• Regulation:
  • expressed under conditions of phosphate limitation (PhoP) PubMed

• Regulatory mechanism:
  • PhoP: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: