Difference between revisions of "YabT"

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(Biological materials)
Line 107: Line 107:
 
** purification from ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP1408, available in [[Stülke]] lab  
 
** purification from ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP1408, available in [[Stülke]] lab  
  
       
 
 
* '''lacZ fusion:'''
 
* '''lacZ fusion:'''
 
* translational lacZ fusion (in [[pAC7]]): pGP831, available in [[Stülke]] lab  
 
* translational lacZ fusion (in [[pAC7]]): pGP831, available in [[Stülke]] lab  

Revision as of 17:02, 8 April 2010

  • Description: similar to Ser/Thr kinase

Gene name yabT
Synonyms
Essential no
Product unknown
Function unknown
MW, pI 37 kDa, 9.943
Gene length, protein length 1014 bp, 338 aa
Immediate neighbours yabS, tilS
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YabT context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU00660

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + a protein = ADP + a phosphoprotein (according to Swiss-Prot)
  • Protein family: protein kinase domain (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation: expressed early during sporulation in the forespore (SigF) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP577 (erm), available in Stülke lab
  • Expression vector:
    • purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP391, available in Stülke lab
    • purification from E. coli with N-terminal Strep-tag, in pGP172: pGP823, available in Stülke lab
    • purification from E. coli with N-terminal His-tag, in pWH844: pGP1408, available in Stülke lab
  • lacZ fusion:
  • translational lacZ fusion (in pAC7): pGP831, available in Stülke lab
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)