Difference between revisions of "List of plasmids"
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Here you can find a list of ''Bacillus subtilis'' plasmids: | Here you can find a list of ''Bacillus subtilis'' plasmids: | ||
| + | ==Fusion to reporter genes== | ||
*[[pAC5]]: translational ''lacZ''-fusions (chloramphenicol resistence) | *[[pAC5]]: translational ''lacZ''-fusions (chloramphenicol resistence) | ||
*[[pAC6]]: transcriptional ''lacZ''-fusions (cat resistence) | *[[pAC6]]: transcriptional ''lacZ''-fusions (cat resistence) | ||
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* pBaSysBioII: gfp transcriptional fusions for high-throughput analysis of gene expression {{PubMed|20150235}} | * pBaSysBioII: gfp transcriptional fusions for high-throughput analysis of gene expression {{PubMed|20150235}} | ||
| + | ==Expression/ purification in ''B. subtilis''== | ||
*[[pBQ200]]: overexpression of proteins in ''B. subtilis'' under control of a strong ''degQ36'' promoter | *[[pBQ200]]: overexpression of proteins in ''B. subtilis'' under control of a strong ''degQ36'' promoter | ||
*[[pGP380]]: expression of proteins in ''B. subtilis'' allows fusion to a Strep-tag at the N-terminus of the protein | *[[pGP380]]: expression of proteins in ''B. subtilis'' allows fusion to a Strep-tag at the N-terminus of the protein | ||
*[[pGP382]]: expression of proteins in ''B. subtilis'' allows fusion to a Strep-tag at the C-terminus of the protein | *[[pGP382]]: expression of proteins in ''B. subtilis'' allows fusion to a Strep-tag at the C-terminus of the protein | ||
| + | ==Expression/ purification in ''E. coli''== | ||
*[[pWH844]]: expression of proteins in ''E. coli'', allows fusion to a N-terminal His-tag | *[[pWH844]]: expression of proteins in ''E. coli'', allows fusion to a N-terminal His-tag | ||
*[[pGP570]]: expression of proteins in ''E. coli'', allows fusion to a N-terminal His-tag that can be cleaved off | *[[pGP570]]: expression of proteins in ''E. coli'', allows fusion to a N-terminal His-tag that can be cleaved off | ||
| − | |||
*[[pGP172]]: expression of proteins in ''E. coli'' allows fusion to a Strep-tag at the N-terminus of the protein | *[[pGP172]]: expression of proteins in ''E. coli'' allows fusion to a Strep-tag at the N-terminus of the protein | ||
*[[pGP574]]: expression of proteins in ''E. coli'' allows fusion to a Strep-tag at the C-terminus of the protein | *[[pGP574]]: expression of proteins in ''E. coli'' allows fusion to a Strep-tag at the C-terminus of the protein | ||
Revision as of 11:46, 11 March 2010
Here you can find a list of Bacillus subtilis plasmids:
Fusion to reporter genes
- pAC5: translational lacZ-fusions (chloramphenicol resistence)
- pAC6: transcriptional lacZ-fusions (cat resistence)
- pAC7: translational lacZ-fusions (kanamycin resistence)
- pBaSysBioII: gfp transcriptional fusions for high-throughput analysis of gene expression PubMed
Expression/ purification in B. subtilis
- pBQ200: overexpression of proteins in B. subtilis under control of a strong degQ36 promoter
- pGP380: expression of proteins in B. subtilis allows fusion to a Strep-tag at the N-terminus of the protein
- pGP382: expression of proteins in B. subtilis allows fusion to a Strep-tag at the C-terminus of the protein
Expression/ purification in E. coli
- pWH844: expression of proteins in E. coli, allows fusion to a N-terminal His-tag
- pGP570: expression of proteins in E. coli, allows fusion to a N-terminal His-tag that can be cleaved off
- pGP172: expression of proteins in E. coli allows fusion to a Strep-tag at the N-terminus of the protein
- pGP574: expression of proteins in E. coli allows fusion to a Strep-tag at the C-terminus of the protein
