Difference between revisions of "PBQ200"

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[[File:PBQ200.jpeg|250px|thumb|right|'''pBQ200: click to enlarge''']]
 
[[File:PBQ200.jpeg|250px|thumb|right|'''pBQ200: click to enlarge''']]
 +
 
'''Application:'''
 
'''Application:'''
 +
The vector was constructed in the G. Rapoport lab and it is suitable for overexpression of proteins in ''B. subtilis''. The plasmid confers resistance to ampicillin and erythromycin in ''E. coli'' and ''B. subtilis'', respectively. pBQ200 is based on the vector pHT315.
  
The vector is suitable for the overexpression of proteins in ''B. subtilis''.
 
 
constructed in the G. Rapoport lab
 
 
in ''E. coli'': ampicillin resistance
 
 
in ''B. subtilis'': erythromycin resistance
 
 
based on [[pHT315]]
 
 
'''Reference:'''
 
'''Reference:'''
 
<pubmed> 8057358 </pubmed>
 
<pubmed> 8057358 </pubmed>

Revision as of 10:59, 25 February 2010

pBQ200: click to enlarge

Application: The vector was constructed in the G. Rapoport lab and it is suitable for overexpression of proteins in B. subtilis. The plasmid confers resistance to ampicillin and erythromycin in E. coli and B. subtilis, respectively. pBQ200 is based on the vector pHT315.

Reference:

I Martin-Verstraete, M Débarbouillé, A Klier, G Rapoport
Interactions of wild-type and truncated LevR of Bacillus subtilis with the upstream activating sequence of the levanase operon.
J Mol Biol: 1994, 241(2);178-92
[PubMed:8057358] [WorldCat.org] [DOI] (P p)