Apt

• Description: adenine phosphoribosyltransferase, universally conserved protein
  
  

Gene name	apt
Synonyms
Essential	no
Product	adenine phosphoribosyltransferase
Function	purine salvage and interconversion
Metabolic function and regulation of this protein in SubtiPathways: Purine salvage, Purine synthesis, Nucleotides (regulation)
MW, pI	18 kDa, 4.701
Gene length, protein length	510 bp, 170 aa
Immediate neighbours	relA, yrvE
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU27610

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides, universally conserved proteins

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: AMP + diphosphate = adenine + 5-phospho-alpha-D-ribose 1-diphosphate (according to Swiss-Prot)

• Protein family: purine/pyrimidine phosphoribosyltransferase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2DY0 (from Escherichia coli k12, 56% identity, 66% similarity)

• UniProt: O34443

• KEGG entry: [3]

• E.C. number: 2.4.2.7

Additional information

universally conserved protein

Expression and regulation

• Operon: yrvE-apt PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

M Nickel, G Homuth, C Böhnisch, U Mäder, T Schweder
Cold induction of the Bacillus subtilis bkd operon is mediated by increased mRNA stability.
Mol Genet Genomics: 2004, 272(1);98-107
[PubMed:15241682] [WorldCat.org] [DOI] (P p)

H H Saxild, P Nygaard
Genetic and physiological characterization of Bacillus subtilis mutants resistant to purine analogs.
J Bacteriol: 1987, 169(7);2977-83
[PubMed:3110131] [WorldCat.org] [DOI] (P p)