Difference between revisions of "MetK"

From SubtiWiki
Jump to: navigation, search
(Database entries)
Line 52: Line 52:
  
  
 +
 +
= Categories containing this gene/protein =
 +
{{SubtiWiki category|[[biosynthesis/ acquisition of amino acids]]}},
 +
{{SubtiWiki category|[[essential genes]]}}
 
=The protein=
 
=The protein=
  

Revision as of 17:56, 30 November 2010

  • Description: S-adenosylmethionine synthetase

Gene name metK
Synonyms metE
Essential yes PubMed
Product S-adenosylmethionine synthetase
Function S-adenosylmethionine synthesis
Metabolic function and regulation of this protein in SubtiPathways:
Cys, Met & Sulfate assimilation
MW, pI 43 kDa, 5.025
Gene length, protein length 1200 bp, 400 aa
Immediate neighbours asnB, pckA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
MetK context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU30550

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, essential genes

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine (according to Swiss-Prot)
  • Protein family: AdoMet synthetase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure: 1FUG (from Escherichia coli, 63% identity, 75% similarity) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion: pGP432 (in pAC7), available in Stülke lab
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Brooke A McDaniel, Frank J Grundy, Vineeta P Kurlekar, Jerneja Tomsic, Tina M Henkin
Identification of a mutation in the Bacillus subtilis S-adenosylmethionine synthetase gene that results in derepression of S-box gene expression.
J Bacteriol: 2006, 188(10);3674-81
[PubMed:16672621] [WorldCat.org] [DOI] (P p)

F J Grundy, T M Henkin
The S box regulon: a new global transcription termination control system for methionine and cysteine biosynthesis genes in gram-positive bacteria.
Mol Microbiol: 1998, 30(4);737-49
[PubMed:10094622] [WorldCat.org] [DOI] (P p)

Alia Lapidus, Nathalie Galleron, Alexei Sorokin, S Dusko Ehrlich
Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region.
Microbiology (Reading): 1997, 143 ( Pt 11);3431-3441
[PubMed:9387221] [WorldCat.org] [DOI] (P p)