FadF

• Description: similar to iron-sulphur-binding reductase
  
  

Gene name	fadF
Synonyms	ywjF
Essential	no
Product	unknown
Function	fatty acid degradation
Metabolic function and regulation of this protein in SubtiPathways: Fatty acid degradation
MW, pI	79 kDa, 6.526
Gene length, protein length	2115 bp, 705 aa
Immediate neighbours	acdA, ywjE
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU37180

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

utilization of lipids, membrane proteins

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): contains an iron-sulfur cluster

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P45866

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: fadF-acdA-rpoE PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed in the absence of long-chain fatty acids (FadR) PubMed
  • repressed by glucose (5-fold) PubMed

• Regulatory mechanism:
  • FadR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Hiroshi Matsuoka, Kazutake Hirooka, Yasutaro Fujita
Organization and function of the YsiA regulon of Bacillus subtilis involved in fatty acid degradation.
J Biol Chem: 2007, 282(8);5180-94
[PubMed:17189250] [WorldCat.org] [DOI] (P p)

Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135] [WorldCat.org] [DOI] (P p)