Difference between revisions of "YloS"
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|style="background:#ABCDEF;" align="center"|'''Function''' || thiamine salvage | |style="background:#ABCDEF;" align="center"|'''Function''' || thiamine salvage | ||
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 23 kDa, 5.216 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 23 kDa, 5.216 | ||
Revision as of 10:40, 16 February 2010
- Description: thiamine pyrophosphokinase
| Gene name | yloS |
| Synonyms | thiN |
| Essential | no |
| Product | thiamine pyrophosphokinase |
| Function | thiamine salvage |
| MW, pI | 23 kDa, 5.216 |
| Gene length, protein length | 642 bp, 214 aa |
| Immediate neighbours | rpe, spoVM |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU15800
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + thiamine = AMP + thiamine diphosphate (according to Swiss-Prot)
- Protein family: thiamine pyrophosphokinase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure: 3CQ9 (from Lactobacillus plantarum, 38% identity, 55% similarity)
- UniProt: O34664
- KEGG entry: [2]
- E.C. number: 2.7.6.2
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Christopher T Jurgenson, Tadhg P Begley, Steven E Ealick
The structural and biochemical foundations of thiamin biosynthesis.
Annu Rev Biochem: 2009, 78;569-603
[PubMed:19348578]
[WorldCat.org]
[DOI]
(I p)
Original publications
Ghislain Schyns, Sébastien Potot, Yi Geng, Teresa M Barbosa, Adriano Henriques, John B Perkins
Isolation and characterization of new thiamine-deregulated mutants of Bacillus subtilis.
J Bacteriol: 2005, 187(23);8127-36
[PubMed:16291685]
[WorldCat.org]
[DOI]
(P p)
Jonathan Melnick, Ewa Lis, Joo-Heon Park, Cynthia Kinsland, Hirotada Mori, Tomoya Baba, John Perkins, Ghislain Schyns, Olga Vassieva, Andrei Osterman, Tadhg P Begley
Identification of the two missing bacterial genes involved in thiamine salvage: thiamine pyrophosphokinase and thiamine kinase.
J Bacteriol: 2004, 186(11);3660-2
[PubMed:15150256]
[WorldCat.org]
[DOI]
(P p)
