Difference between revisions of "ThiO"

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|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of thiamine
 
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of thiamine
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/thiamin.html Thiamin synthesis]'''
 
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 40 kDa, 5.898   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 40 kDa, 5.898   

Revision as of 10:47, 16 February 2010

  • Description: FAD-dependent glycine oxidase

Gene name thiO
Synonyms goxB, yjbR
Essential no
Product glycine oxidase
Function biosynthesis of thiamine
MW, pI 40 kDa, 5.898
Gene length, protein length 1107 bp, 369 aa
Immediate neighbours tenI, thiS
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
GoxB context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU11670

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Glycine + H2O + O2 = glyoxylate + NH3 + H2O2 (according to Swiss-Prot)
  • Protein family: DAMOX/DASOX family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions: the FAD-containing holoenzyme is a homotetramer PubMed
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure: 1NG3 (complex with acetyl-glycine), 1RYI
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Christopher T Jurgenson, Tadhg P Begley, Steven E Ealick
The structural and biochemical foundations of thiamin biosynthesis.
Annu Rev Biochem: 2009, 78;569-603
[PubMed:19348578] [WorldCat.org] [DOI] (I p)

Original publications