Difference between revisions of "DnaI"
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| − | * '''Description:''' primosome component (helicase loader) <br/><br/> | + | * '''Description:''' primosome component (helicase loader), part of the [[replisome]] <br/><br/> |
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* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
| − | * '''Interactions:''' [[DnaB]]-[[DnaI]], [[DnaC]]-[[DnaI]] [http://www.ncbi.nlm.nih.gov/sites/entrez/10844689 PubMed] | + | * '''Interactions:''' [[DnaB]]-[[DnaI]], [[DnaC]]-[[DnaI]] [http://www.ncbi.nlm.nih.gov/sites/entrez/10844689 PubMed], part of the [[replisome]]: [[PolC]]-[[HolA]]-[[HolB]]-[[DnaX]]-[[DnaN]]-[[DnaG]]-[[DnaC]]-[[DnaI]]-[[DnaD]]-[[SsbA]]-[[DnaE]]-[[PriA]]-[[DnaB]] {{PubMed|20122408}} |
* '''Localization:''' cell membrane (according to Swiss-Prot), Cytoplasm (Homogeneous) [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed] forms foci, close to oriC [http://www.ncbi.nlm.nih.gov/sites/entrez/10844689 PubMed] | * '''Localization:''' cell membrane (according to Swiss-Prot), Cytoplasm (Homogeneous) [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed] forms foci, close to oriC [http://www.ncbi.nlm.nih.gov/sites/entrez/10844689 PubMed] | ||
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=References= | =References= | ||
| − | <pubmed>19255093,10844689,12718886,11842108,, 16479537, 19744498 , 19255093 19968790 11679082 </pubmed> | + | <pubmed>19255093,10844689,12718886,11842108, 20122408, 16479537, 19744498 , 19255093 19968790 11679082 </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 09:24, 4 February 2010
- Description: primosome component (helicase loader), part of the replisome
| Gene name | dnaI |
| Synonyms | ytxA, dnaY |
| Essential | yes PubMed |
| Product | primosome component (helicase loader) |
| Function | DNA replication |
| MW, pI | 35 kDa, 5.743 |
| Gene length, protein length | 933 bp, 311 aa |
| Immediate neighbours | ytxB, dnaB |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU28980
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions: DnaB-DnaI, DnaC-DnaI PubMed, part of the replisome: PolC-HolA-HolB-DnaX-DnaN-DnaG-DnaC-DnaI-DnaD-SsbA-DnaE-PriA-DnaB PubMed
- Localization: cell membrane (according to Swiss-Prot), Cytoplasm (Homogeneous) PubMed forms foci, close to oriC PubMed
Database entries
- Structure: 2K7R (N-terminal domain)
- UniProt: P06567
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Glenn M Sanders, H Garry Dallmann, Charles S McHenry
Reconstitution of the B. subtilis replisome with 13 proteins including two distinct replicases.
Mol Cell: 2010, 37(2);273-81
[PubMed:20122408]
[WorldCat.org]
[DOI]
(I p)
Wiep Klaas Smits, Alexi I Goranov, Alan D Grossman
Ordered association of helicase loader proteins with the Bacillus subtilis origin of replication in vivo.
Mol Microbiol: 2010, 75(2);452-61
[PubMed:19968790]
[WorldCat.org]
[DOI]
(I p)
Kuang-Lei Tsai, Yu-Hua Lo, Yuh-Ju Sun, Chwan-Deng Hsiao
Molecular interplay between the replicative helicase DnaC and its loader protein DnaI from Geobacillus kaustophilus.
J Mol Biol: 2009, 393(5);1056-69
[PubMed:19744498]
[WorldCat.org]
[DOI]
(I p)
Karin V Loscha, Kristaps Jaudzems, Charikleia Ioannou, Xun-Cheng Su, Flynn R Hill, Gottfried Otting, Nicholas E Dixon, Edvards Liepinsh
A novel zinc-binding fold in the helicase interaction domain of the Bacillus subtilis DnaI helicase loader.
Nucleic Acids Res: 2009, 37(7);2395-404
[PubMed:19255093]
[WorldCat.org]
[DOI]
(I p)
Jean-Christophe Meile, Ling Juan Wu, S Dusko Ehrlich, Jeff Errington, Philippe Noirot
Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory.
Proteomics: 2006, 6(7);2135-46
[PubMed:16479537]
[WorldCat.org]
[DOI]
(P p)
Marion Velten, Stephen McGovern, Stéphanie Marsin, S Dusko Ehrlich, Philippe Noirot, Patrice Polard
A two-protein strategy for the functional loading of a cellular replicative DNA helicase.
Mol Cell: 2003, 11(4);1009-20
[PubMed:12718886]
[WorldCat.org]
[DOI]
(P p)
P Soultanas
A functional interaction between the putative primosomal protein DnaI and the main replicative DNA helicase DnaB in Bacillus.
Nucleic Acids Res: 2002, 30(4);966-74
[PubMed:11842108]
[WorldCat.org]
[DOI]
(I p)
C Bruand, M Farache, S McGovern, S D Ehrlich, P Polard
DnaB, DnaD and DnaI proteins are components of the Bacillus subtilis replication restart primosome.
Mol Microbiol: 2001, 42(1);245-55
[PubMed:11679082]
[WorldCat.org]
[DOI]
(P p)
Y Imai, N Ogasawara, D Ishigo-Oka, R Kadoya, T Daito, S Moriya
Subcellular localization of Dna-initiation proteins of Bacillus subtilis: evidence that chromosome replication begins at either edge of the nucleoids.
Mol Microbiol: 2000, 36(5);1037-48
[PubMed:10844689]
[WorldCat.org]
[DOI]
(P p)
