YclN

• Description: petrobactin (3.4-catecholate siderophore) ABC transporter (permease)
  
  

Gene name	yclN
Synonyms
Essential	no
Product	petrobactin ABC transporter (permease)
Function	iron acquisition
MW, pI	34 kDa, 9.817
Gene length, protein length	948 bp, 316 aa
Immediate neighbours	yclM, yclO
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU03800

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: uptake of the siderophore petrobactin PubMed

• Protein family: FecCD subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P94418

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: yclN-yclO-yclP-yclQ PubMed

• Sigma factor:

• Regulation:
  • induced upon iron starvation (Fur) PubMed

• Regulatory mechanism:
  • Fur: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Anna M Zawadzka, Youngchang Kim, Natalia Maltseva, Rita Nichiporuk, Yao Fan, Andrzej Joachimiak, Kenneth N Raymond
Characterization of a Bacillus subtilis transporter for petrobactin, an anthrax stealth siderophore.
Proc Natl Acad Sci U S A: 2009, 106(51);21854-9
[PubMed:19955416] [WorldCat.org] [DOI] (I p)

Noel Baichoo, Tao Wang, Rick Ye, John D Helmann
Global analysis of the Bacillus subtilis Fur regulon and the iron starvation stimulon.
Mol Microbiol: 2002, 45(6);1613-29
[PubMed:12354229] [WorldCat.org] [DOI] (P p)

Y Quentin, G Fichant, F Denizot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J Mol Biol: 1999, 287(3);467-84
[PubMed:10092453] [WorldCat.org] [DOI] (P p)