CitS

• Description: two-component sensor kinase, regulation of citrate uptake
  
  

Gene name	citS
Synonyms	yflR
Essential	no
Product	two-component sensor kinase
Function	regulation of citrate uptake
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	59 kDa, 6.481
Gene length, protein length	1626 bp, 542 aa
Immediate neighbours	yflS, citT
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU07580

Phenotypes of a mutant

no growth with citrate as sole carbon source PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

utilization of specific carbon sources, protein modification, transcription factors and their control, membrane proteins, phosphoproteins

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: autophosphorylation, phosphorylation of CitT

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains: two transmembrane segments, C-terminal histidine phosphotransferase domain

• Modification: autophosphorylation on a His residue

• Cofactor(s):

• Effectors of protein activity:

• Interactions: CitS-CitT

• Localization: cell membrane (according to Swiss-Prot), Membrane (Heterogeneous) PubMed

Database entries

• Structure:

• UniProt: O34427

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: citS-citT-yflP PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: