MrnC

• Description: RNase Mini-III
  
  

Gene name	mrnC
Synonyms	yazC
Essential	no
Product	RNase Mini-III
Function	maturation of 23S rRNA
Metabolic function and regulation of this protein in SubtiPathways: tRNA charging
MW, pI	16 kDa, 9.153
Gene length, protein length	429 bp, 143 aa
Immediate neighbours	cysS, yacO
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU00950

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

Rnases, translation

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: RNase III family (but lacks the dsRNA-binding domain)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity: RplC binding to the precursor 23S rRNA stimulates MrnC activity PubMed

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O31418

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: gltX-cysE-cysS-mrnC-yacO-yacP PubMed

• Sigma factor: SigA PubMed

• Regulation: repression by cysteine (T-box) PubMed

• Regulatory mechanism: T-box, overlaps a transcription terminator upstream of cysE: transcription antitermination PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Ciaran Condon, IBPC, Paris, France Homepage

David Bechhofer, Mount Sinai School, New York, USA Homepage