Difference between revisions of "IlvB"
| Line 95: | Line 95: | ||
* '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/1577690 PubMed] | * '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/1577690 PubMed] | ||
| − | * '''Regulation:''' | + | * '''Regulation:''' for a complete overview on the regulation of the ilv operon, see [http://www.ncbi.nlm.nih.gov/pubmed/20935095 Brinsmade ''et al''.] |
** expression is stimulated in the presence of glucose ([[CcpA]]) [http://www.ncbi.nlm.ntnrih.gov/sites/entrez/12193635 PubMed] | ** expression is stimulated in the presence of glucose ([[CcpA]]) [http://www.ncbi.nlm.ntnrih.gov/sites/entrez/12193635 PubMed] | ||
** repressed under nitrogen limiting conditions (glutamine or ammonium) ([[TnrA]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/15547269 PubMed] | ** repressed under nitrogen limiting conditions (glutamine or ammonium) ([[TnrA]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/15547269 PubMed] | ||
| Line 129: | Line 129: | ||
=References= | =References= | ||
| − | <pubmed>15060025,19258532,8289305,17488331,18083814,18641142,15547269,12618455,,15547269,12618455,12107147 1577690 12193635 15916605 15916606 15547269, </pubmed> | + | <pubmed>15060025,19258532,8289305,17488331,18083814,18641142,15547269,12618455,,15547269,12618455,12107147 1577690 12193635 15916605 15916606 15547269, 20935095</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 09:28, 26 October 2010
- Description: acetolactate synthase (large subunit)
| Gene name | ilvB |
| Synonyms | |
| Essential | no |
| Product | acetolactate synthase (large subunit) |
| Function | biosynthesis of branched-chain amino acids |
| Metabolic function and regulation of this protein in SubtiPathways: Ile, Leu, Val, Coenzyme A | |
| MW, pI | 62 kDa, 5.178 |
| Gene length, protein length | 1722 bp, 574 aa |
| Immediate neighbours | ilvH, ysnD |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU28310
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: 2 pyruvate = 2-acetolactate + CO2 (according to Swiss-Prot)
- Protein family: TPP enzyme family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure: 1N53 (T box RNA)
- UniProt: P37251
- KEGG entry: [3]
- E.C. number: 2.2.1.6
Additional information
- subject to Clp-dependent proteolysis upon glucose starvation PubMed
Expression and regulation
- Regulation: for a complete overview on the regulation of the ilv operon, see Brinsmade et al.
- Regulatory mechanism:
- Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed
Biological materials
- Mutant: GP324, ilvB under control of pXyl (cat), available in Jörg Stülke lab
- Expression vector:
- lacZ fusion: pGP520 and pGP524 (both in pAC5), available in Stülke lab; pGP523 (in pAC6), available in Stülke lab
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
