Difference between revisions of "GlpF"
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* '''Operon:''' ''[[glpF]]-[[glpK]]'' {{PubMed|2127799}} | * '''Operon:''' ''[[glpF]]-[[glpK]]'' {{PubMed|2127799}} | ||
| − | * '''[[Sigma factor]]:''' [[SigA]] | + | * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|2127799}} |
* '''Regulation:''' | * '''Regulation:''' | ||
| − | ** repressed by glucose (8-fold) ([[CcpA]]) | + | ** repressed by glucose (8-fold) ([[CcpA]]) {{PubMed|12850135}} |
| − | ** induction by glycerol ([[GlpP]]) | + | ** induction by glycerol ([[GlpP]]) {{PubMed|8436953}} |
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
| − | ** [[CcpA]]: transcription repression | + | ** [[CcpA]]: transcription repression {{PubMed|12850135}} |
| − | ** [[GlpP]]-dependent [[RNA switch]] (transcriptional antitermination) | + | ** [[GlpP]]-dependent [[RNA switch]] (transcriptional antitermination) {{PubMed|8436953}} |
* '''Additional information:''' | * '''Additional information:''' | ||
Revision as of 18:53, 4 September 2009
- Description: glycerol facilitator
| Gene name | glpF |
| Synonyms | |
| Essential | no |
| Product | glycerol facilitator |
| Function | glycerol uptake |
| Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism | |
| MW, pI | 28 kDa, 9.506 |
| Gene length, protein length | 822 bp, 274 aa |
| Immediate neighbours | glpP, glpK |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU09280
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: View classification (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: cell membrane (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: P18156
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulatory mechanism:
- CcpA: transcription repression PubMed
- GlpP-dependent RNA switch (transcriptional antitermination) PubMed
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135]
[WorldCat.org]
[DOI]
(P p)
Emmanuelle Darbon, Pascale Servant, Sandrine Poncet, Josef Deutscher
Antitermination by GlpP, catabolite repression via CcpA and inducer exclusion triggered by P-GlpK dephosphorylation control Bacillus subtilis glpFK expression.
Mol Microbiol: 2002, 43(4);1039-52
[PubMed:11929549]
[WorldCat.org]
[DOI]
(P p)
L Beijer, R P Nilsson, C Holmberg, L Rutberg
The glpP and glpF genes of the glycerol regulon in Bacillus subtilis.
J Gen Microbiol: 1993, 139(2);349-59
[PubMed:8436953]
[WorldCat.org]
[DOI]
(P p)
