Difference between revisions of "Cah"

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(Expression and regulation)
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= Categories containing this gene/protein =
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{{SubtiWiki category|[[resistance against toxins/ antibiotics]]}}
 
=The protein=
 
=The protein=
  

Revision as of 15:32, 30 November 2010

  • Description: cephalosporin C deacetylase

Gene name cah
Synonyms
Essential no
Product cephalosporin C deacetylase)
Function resistance to cephalosporin C
MW, pI 35 kDa, 5.419
Gene length, protein length 954 bp, 318 aa
Immediate neighbours ycgK, ycgL
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Cah context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU03180

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

Categories containing this gene/protein

resistance against toxins/ antibiotics

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Deacetylation of xylans and xylo-oligosaccharides (according to Swiss-Prot)
  • Protein family: carbohydrate esterase 7 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

A Takimoto, S Yagi, K Mitsushima
High-level expression, purification, and some properties of a recombinant cephalosporin-C deacetylase.
J Biosci Bioeng: 1999, 87(4);456-62
[PubMed:16232499] [WorldCat.org] [DOI] (P p)

Akio Takimoto, Tomoaki Takakura, Hiroyoshi Tani, Shigeo Yagi, Kenji Mitsushima
Batch production of deacetyl 7-aminocephalosporanic acid by immobilized cephalosporin-C deacetylase.
Appl Microbiol Biotechnol: 2004, 65(3);263-7
[PubMed:15069587] [WorldCat.org] [DOI] (P p)

Florence Vincent, Simon J Charnock, Koen H G Verschueren, Johan P Turkenburg, David J Scott, Wendy A Offen, Shirley Roberts, Gavin Pell, Harry J Gilbert, Gideon J Davies, James A Brannigan
Multifunctional xylooligosaccharide/cephalosporin C deacetylase revealed by the hexameric structure of the Bacillus subtilis enzyme at 1.9A resolution.
J Mol Biol: 2003, 330(3);593-606
[PubMed:12842474] [WorldCat.org] [DOI] (P p)

K Mitsushima, A Takimoto, T Sonoyama, S Yagi
Gene cloning, nucleotide sequence, and expression of a cephalosporin-C deacetylase from Bacillus subtilis.
Appl Environ Microbiol: 1995, 61(6);2224-9
[PubMed:7793942] [WorldCat.org] [DOI] (P p)