Difference between revisions of "RsfA"

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* '''Description:''' probable regulator of transcription of SigF-dependent genes <br/><br/>
+
* '''Description:''' probable regulator of transcription of [[SigF]]-dependent genes <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || regulator of SigF activity
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|style="background:#ABCDEF;" align="center"| '''Product''' || regulator of [[SigF]] activity
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || control of expression of SigF-dependent genes
+
|style="background:#ABCDEF;" align="center"|'''Function''' || control of expression of [[SigF]]-dependent genes
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 29 kDa, 5.13   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 29 kDa, 5.13   
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* '''Operon:''' ''rsfA''
 
* '''Operon:''' ''rsfA''
  
* '''[[Sigma factor]]:''' [[SigF]] {{PubMed|15699190}}
+
* '''[[Sigma factor]]:''' [[SigF]] {{PubMed|16497325}}
  
* '''Regulation:''' repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed]   
+
* '''Regulation:'''  
 +
** expressed early during sporulation in the forespore ([[SigF]]) {{PubMed|16497325,15699190}}
 +
** repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed]   
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  

Revision as of 12:18, 1 November 2009

  • Description: probable regulator of transcription of SigF-dependent genes

Gene name rsfA
Synonyms ywfN, ipa-92r
Essential no
Product regulator of SigF activity
Function control of expression of SigF-dependent genes
MW, pI 29 kDa, 5.13
Gene length, protein length 774 bp, 258 aa
Immediate neighbours ywzC, ywfM
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
RsfA context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU37620

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: forespore (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon: rsfA
  • Regulation:
    • expressed early during sporulation in the forespore (SigF) PubMed
    • repressed by casamino acids PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)

L J Wu, J Errington
Identification and characterization of a new prespore-specific regulatory gene, rsfA, of Bacillus subtilis.
J Bacteriol: 2000, 182(2);418-24
[PubMed:10629188] [WorldCat.org] [DOI] (P p)