Difference between revisions of "HemN"

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(Expression and regulation)
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* '''Operon:''' ''[[lepA]]-[[hemN]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/8757728 PubMed]
 
* '''Operon:''' ''[[lepA]]-[[hemN]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/8757728 PubMed]
  
* '''[[Sigma factor]]:''' [[SigE]] {{PubMed|15699190}}
+
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|9371469}}
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  

Revision as of 18:28, 22 November 2009

  • Description: anaerobic coproporphyrinogen III oxidase

Gene name hemN
Synonyms yqeR
Essential no
Product anaerobic coproporphyrinogen III oxidase
Function heme biosynthesis
MW, pI 41 kDa, 5.534
Gene length, protein length 1098 bp, 366 aa
Immediate neighbours hrcA, lepA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
HemN context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU25500

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Coproporphyrinogen-III + 2 S-adenosyl-L-methionine = protoporphyrinogen-IX + 2 CO2 + 2 L-methionine + 2 5'-deoxyadenosine (according to Swiss-Prot)
  • Protein family: anaerobic coproporphyrinogen III oxidase family (according to Swiss-Prot)
  • Paralogous protein(s): HemZ

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information: There is a terminator between lepA and hemN with only little readthrough

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

G Homuth, A Rompf, W Schumann, D Jahn
Transcriptional control of Bacillus subtilis hemN and hemZ.
J Bacteriol: 1999, 181(19);5922-9
[PubMed:10498703] [WorldCat.org] [DOI] (P p)

B Hippler, G Homuth, T Hoffmann, C Hungerer, W Schumann, D Jahn
Characterization of Bacillus subtilis hemN.
J Bacteriol: 1997, 179(22);7181-5
[PubMed:9371469] [WorldCat.org] [DOI] (P p)

G Homuth, M Heinemann, U Zuber, W Schumann
The genes of lepA and hemN form a bicistronic operon in Bacillus subtilis.
Microbiology (Reading): 1996, 142 ( Pt 7);1641-9
[PubMed:8757728] [WorldCat.org] [DOI] (P p)