Difference between revisions of "PrkC"

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(Biological materials)
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=Biological materials =
 
=Biological materials =
  
* '''Mutant:'''
+
* '''Mutant:''' GP576 (spc), OMG302 (aphA3), available in [[Stülke]] lab
  
* '''Expression vector:''' pGP832 (N-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP380]]), available in [[Stülke]] lab
+
* '''Expression vector:'''  
       
+
** pGP832 (N-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP380]]), available in [[Stülke]] lab
 +
** for expression, purification in ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP1001,  available in [[Stülke]] lab
 +
** for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [[pGP172]]: pGP825,  available in [[Stülke]] lab
 +
                                       
 
* '''lacZ fusion:'''
 
* '''lacZ fusion:'''
  

Revision as of 16:09, 2 September 2009

  • Description: protein kinase C

Gene name prkC
Synonyms yloP
Essential no
Product protein kinase
Function germination in response to muropeptides
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 71 kDa, 4.833
Gene length, protein length 1944 bp, 648 aa
Immediate neighbours prpC, cpgA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
PrkC context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU15770

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + a protein = ADP + a phosphoprotein (according to Swiss-Prot)
  • Protein family: protein kinase domain (according to Swiss-Prot)
  • Paralogous protein(s):

Proteins phosphorylated by PrkC

CpgA, EF-Tu, YezB PubMed, EF-G PubMed

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylation on Thr-290 PubMed
  • Cofactor(s):
  • Effectors of protein activity: activated by muropeptides PubMed
  • Interactions:
  • Localization: membrane (according to Swiss-Prot), membrane PubMed

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP576 (spc), OMG302 (aphA3), available in Stülke lab
  • Expression vector:
    • pGP832 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab
    • for expression, purification in E. coli with N-terminal His-tag, in pWH844: pGP1001, available in Stülke lab
    • for expression, purification in E. coli with N-terminal Strep-tag, in pGP172: pGP825, available in Stülke lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References