GntK

• Description: gluconokinase
  
  

Gene name	gntK
Synonyms
Essential	no
Product	gluconokinase
Function	gluconate utilization
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	56 kDa, 5.517
Gene length, protein length	1539 bp, 513 aa
Immediate neighbours	gntR, gntP
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU40060

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + D-gluconate = ADP + 6-phospho-D-gluconate (according to Swiss-Prot)

• Protein family: FGGY kinase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P12011

• KEGG entry: [3]

• E.C. number: 2.7.1.12

Expression and regulation

• Operon: gntR-gntK-gntP-gntZ PubMed1PubMed2

• Sigma factor: SigA PubMed

• Regulation:
  • induced by gluconate (GntR) PubMed
  • repressed by glucose (CcpA) PubMed

• Regulatory mechanism:
  • GntR: transcription repression PubMed
  • CcpA: transcription repression PubMed
  • AbrB: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Onuma Chumsakul, Hiroki Takahashi, Taku Oshima, Takahiro Hishimoto, Shigehiko Kanaya, Naotake Ogasawara, Shu Ishikawa
Genome-wide binding profiles of the Bacillus subtilis transition state regulator AbrB and its homolog Abh reveals their interactive role in transcriptional regulation.
Nucleic Acids Res: 2011, 39(2);414-28
[PubMed:20817675] [WorldCat.org] [DOI] (I p)

Ken-Ichi Yoshida, Izumi Ishio, Eishi Nagakawa, Yoshiyuki Yamamoto, Mami Yamamoto, Yasutaro Fujita
Systematic study of gene expression and transcription organization in the gntZ-ywaA region of the Bacillus subtilis genome.
Microbiology (Reading): 2000, 146 ( Pt 3);573-579
[PubMed:10746760] [WorldCat.org] [DOI] (P p)

Y Fujita, Y Miwa
Catabolite repression of the Bacillus subtilis gnt operon mediated by the CcpA protein.
J Bacteriol: 1994, 176(2);511-3
[PubMed:8288545] [WorldCat.org] [DOI] (P p)

Y Miwa, Y Fujita
Promoter-independent catabolite repression of the Bacillus subtilis gnt operon.
J Biochem: 1993, 113(6);665-71
[PubMed:8370661] [WorldCat.org] [DOI] (P p)

A Reizer, J Deutscher, M H Saier, J Reizer
Analysis of the gluconate (gnt) operon of Bacillus subtilis.
Mol Microbiol: 1991, 5(5);1081-9
[PubMed:1659648] [WorldCat.org] [DOI] (P p)

Y Fujita, T Fujita
Effect of mutations causing gluconate kinase or gluconate permease deficiency on expression of the Bacillus subtilis gnt operon.
J Bacteriol: 1989, 171(3);1751-4
[PubMed:2537826] [WorldCat.org] [DOI] (P p)

Y Fujita, T Fujita
The gluconate operon gnt of Bacillus subtilis encodes its own transcriptional negative regulator.
Proc Natl Acad Sci U S A: 1987, 84(13);4524-8
[PubMed:3037520] [WorldCat.org] [DOI] (P p)

Y Fujita, T Fujita, Y Miwa, J Nihashi, Y Aratani
Organization and transcription of the gluconate operon, gnt, of Bacillus subtilis.
J Biol Chem: 1986, 261(29);13744-53
[PubMed:3020045] [WorldCat.org] (P p)

Y Fujita, J Nihashi, T Fujita
The characterization and cloning of a gluconate (gnt) operon of Bacillus subtilis.
J Gen Microbiol: 1986, 132(1);161-9
[PubMed:3011959] [WorldCat.org] [DOI] (P p)

J Nihashi, Y Fujita
Catabolite repression of inositol dehydrogenase and gluconate kinase syntheses in Bacillus subtilis.
Biochim Biophys Acta: 1984, 798(1);88-95
[PubMed:6322857] [WorldCat.org] [DOI] (P p)