Difference between revisions of "UreC"

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(Expression and regulation)
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* '''[[Sigma factor]]:''' [[SigA]], [[SigH]] [http://www.ncbi.nlm.nih.gov/sites/entrez/9287005 PubMed]
 
* '''[[Sigma factor]]:''' [[SigA]], [[SigH]] [http://www.ncbi.nlm.nih.gov/sites/entrez/9287005 PubMed]
  
* '''Regulation:''' induced by nitrogen limitation ([[GlnR]], [[TnrA]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/9287005 PubMed], repressed by [[CodY]] [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed]
+
* '''Regulation:'''  
** expressed in the absence of good nitrogen sources (glutamine or ammonium) ([[TnrA]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/9287005 PubMed]
+
** induced by nitrogen limitation ([[GlnR]], [[TnrA]]) {{PubMed|9287005}}
 
** induced in the presence of purine nucleotides (inducer: allantoin) ([[PucR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12374841 PubMed]
 
** induced in the presence of purine nucleotides (inducer: allantoin) ([[PucR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12374841 PubMed]
** repressed during growth in the presence of branched chain amino acids ([[CodY]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/9287005 PubMed]
+
** repressed during growth in the presence of branched chain amino acids ([[CodY]]) {{PubMed|9287005,12618455}}
  
* '''Regulatory mechanism:''' [[GlnR]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/9287005 PubMed], [[TnrA]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/9287005 PubMed], [[CodY]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed] 
+
* '''Regulatory mechanism:'''  
** [[TnrA]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/9287005 PubMed]
+
** [[GlnR]]: transcription repression {{PubMed|9287005}}
** [PucR]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/12374841 PubMed]
+
** [[TnrA]]: transcription activation {{PubMed|9287005}}
** [[CodY]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/9287005 PubMed]
+
** [[PucR]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/12374841 PubMed]
 +
** [[CodY]]: transcription repression {{PubMed|9287005,12618455}}
  
 
* '''Additional information:'''
 
* '''Additional information:'''

Revision as of 19:16, 2 February 2010

  • Description: urease (alpha subunit)

Gene name ureC
Synonyms
Essential no
Product urease (alpha subunit)
Function utilization of urea as alternative nitrogen source
Metabolic function and regulation of this protein in SubtiPathways:
Purine catabolism, Ammonium/ glutamate
MW, pI 61 kDa, 5.091
Gene length, protein length 1707 bp, 569 aa
Immediate neighbours ywnA, ureB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
UreC context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU36640

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Urea + H2O = CO2 + 2 NH3 (according to Swiss-Prot)
  • Protein family: urease family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • induced by nitrogen limitation (GlnR, TnrA) PubMed
    • induced in the presence of purine nucleotides (inducer: allantoin) (PucR) PubMed
    • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Virginie Molle, Yoshiko Nakaura, Robert P Shivers, Hirotake Yamaguchi, Richard Losick, Yasutaro Fujita, Abraham L Sonenshein
Additional targets of the Bacillus subtilis global regulator CodY identified by chromatin immunoprecipitation and genome-wide transcript analysis.
J Bacteriol: 2003, 185(6);1911-22
[PubMed:12618455] [WorldCat.org] [DOI] (P p)

Jaclyn L Brandenburg, Lewis V Wray, Lars Beier, Hanne Jarmer, Hans H Saxild, Susan H Fisher
Roles of PucR, GlnR, and TnrA in regulating expression of the Bacillus subtilis ure P3 promoter.
J Bacteriol: 2002, 184(21);6060-4
[PubMed:12374841] [WorldCat.org] [DOI] (P p)

L V Wray, A E Ferson, S H Fisher
Expression of the Bacillus subtilis ureABC operon is controlled by multiple regulatory factors including CodY, GlnR, TnrA, and Spo0H.
J Bacteriol: 1997, 179(17);5494-501
[PubMed:9287005] [WorldCat.org] [DOI] (P p)