Lgt

• Description: prolipoprotein diacylglyceryl transferase
  
  

Gene name	lgt
Synonyms	gerF, yvoC
Essential	no
Product	prolipoprotein diacylglyceryl transferase
Function	lipomodification of lipoproteins
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	30 kDa, 8.96
Gene length, protein length	807 bp, 269 aa
Immediate neighbours	yvoD, hprK
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU34990

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: lgt family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot), membrane associated PubMed

Database entries

• Structure:

• UniProt: O34752

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP851 (deletion of lgt-yvoD-yvoE-yvoF, replaced by aphA3), available in the Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Takao Igarashi, Barbara Setlow, Madan Paidhungat, Peter Setlow
Effects of a gerF (lgt) mutation on the germination of spores of Bacillus subtilis.
J Bacteriol: 2004, 186(10);2984-91
[PubMed:15126458] [WorldCat.org] [DOI] (P p)

S Leskelä, E Wahlström, V P Kontinen, M Sarvas
Lipid modification of prelipoproteins is dispensable for growth but essential for efficient protein secretion in Bacillus subtilis: characterization of the Lgt gene.
Mol Microbiol: 1999, 31(4);1075-85
[PubMed:10096076] [WorldCat.org] [DOI] (P p)

J Bengtsson, H Tjalsma, C Rivolta, L Hederstedt
Subunit II of Bacillus subtilis cytochrome c oxidase is a lipoprotein.
J Bacteriol: 1999, 181(2);685-8
[PubMed:9882689] [WorldCat.org] [DOI] (P p)