Tgl

• Description: spore associated transglutaminase
  
  

Gene name	tgl
Synonyms	yuxF, yugV
Essential	no
Product	spore associated transglutaminase
Function	introduction of crosslinks in the spore coat protein GerQ
MW, pI	28 kDa, 6.687
Gene length, protein length	735 bp, 245 aa
Immediate neighbours	mcpB, yugU
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU31270

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Protein glutamine + alkylamine = protein N(5)-alkylglutamine + NH₃ (according to Swiss-Prot)

• Protein family: bacillus TGase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P40746

• KEGG entry: [3]

• E.C. number: 2.3.2.13

Expression and regulation

• Operon:

• Sigma factor: SigK PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Adriano Henriques, Lisbon, Portugal homepage

References

Diana Plácido, Catarina G Fernandes, Anabela Isidro, Maria Arménia Carrondo, Adriano O Henriques, Margarida Archer
Auto-induction and purification of a Bacillus subtilis transglutaminase (Tgl) and its preliminary crystallographic characterization.
Protein Expr Purif: 2008, 59(1);1-8
[PubMed:18249137] [WorldCat.org] [DOI] (I p)

Rita Zilhão, Rachele Isticato, Lígia O Martins, Leif Steil, Uwe Völker, Ezio Ricca, Charles P Moran, Adriano O Henriques
Assembly and function of a spore coat-associated transglutaminase of Bacillus subtilis.
J Bacteriol: 2005, 187(22);7753-64
[PubMed:16267299] [WorldCat.org] [DOI] (P p)

Katsunori Kobayashi, Shun-Ichi Suzuki, Yuko Izawa, Kiyoshi Miwa, Shigeru Yamanaka
Transglutaminase in sporulating cells of Bacillus subtilis.
J Gen Appl Microbiol: 1998, 44(1);85-91
[PubMed:12501297] [WorldCat.org] [DOI] (I p)

S Suzuki, Y Izawa, K Kobayashi, Y Eto, S Yamanaka, K Kubota, K Yokozeki
Purification and characterization of novel transglutaminase from Bacillus subtilis spores.
Biosci Biotechnol Biochem: 2000, 64(11);2344-51
[PubMed:11193401] [WorldCat.org] [DOI] (P p)