Difference between revisions of "PheT"

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=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:'''  
+
* '''Operon:''' ''[[pheS]]-[[pheT]]'' {{PubMed|2127701}}
  
* '''[[Sigma factor]]:'''  
+
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|2127701}}
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
 +
** induced by phenylalanine limitation ([[T-box]]) {{PubMed|19258532}}
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
 
+
** [[T-box]]: [[RNA switch]], transcriptional antitermination {{PubMed|19258532}}
* '''Additional information:'''
 
  
 
=Biological materials =
 
=Biological materials =
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=References=
 
=References=
  
<pubmed>19258532,, </pubmed>
+
<pubmed>19258532,2127701, </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 20:05, 1 October 2009

  • Description: phenylalanyl-tRNA synthetase (beta subunit)

Gene name pheT
Synonyms
Essential yes PubMed
Product phenylalanyl-tRNA synthetase (beta subunit)
Function translation
Metabolic function and regulation of this protein in SubtiPathways:
tRNA charging
MW, pI 87 kDa, 4.864
Gene length, protein length 2412 bp, 804 aa
Immediate neighbours rnhC, pheS
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
PheT context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU28630

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe) (according to Swiss-Prot)
  • Protein family: Type 1 subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)

A A Brakhage, M Wozny, H Putzer
Structure and nucleotide sequence of the Bacillus subtilis phenylalanyl-tRNA synthetase genes.
Biochimie: 1990, 72(10);725-34
[PubMed:2127701] [WorldCat.org] [DOI] (P p)