Difference between revisions of "RibE"

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(Biological materials)
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=Biological materials =
 
=Biological materials =
  
* '''Mutant:'''
+
* '''Mutant:''' GP203 (erm), available in the [[Stülke]] lab
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''

Revision as of 17:30, 30 September 2009

  • Description: riboflavin synthase (alpha subunit)

Gene name ribE
Synonyms ribB
Essential no
Product riboflavin synthase (alpha subunit)
Function riboflavin biosynthesis
MW, pI 23 kDa, 5.845
Gene length, protein length 645 bp, 215 aa
Immediate neighbours ribA, ribD
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
RibE context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU23270

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine (according to Swiss-Prot)
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulatory mechanism: FMN-box: riboswitch, mediates termination/ antitermination control of the operon, in the absence of FMN: antitermination, in the presence of FMN: termination PubMed
  • Additional information:

Biological materials

  • Mutant: GP203 (erm), available in the Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References