Difference between revisions of "YjiB"

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* '''Description:''' similar to monooxygenase <br/><br/>
+
* '''Description:''' monooxygenase CYP109B1 <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown
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|style="background:#ABCDEF;" align="center"| '''Product''' || monooxygenase CYP109B1
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
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|style="background:#ABCDEF;" align="center"|'''Function''' || oxidation of fatty acids
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 44 kDa, 5.379   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 44 kDa, 5.379   
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=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
* '''Catalyzed reaction/ biological activity:'''  
+
* '''Catalyzed reaction/ biological activity:''' oxidation of saturated fatty acids (conversion up to 99%) and their methyl and ethyl esters (conversion up to 80%) at subterminal positions with a preference for the carbon atoms C11 and C12 counted from the carboxyl group {{PubMed|20186410}}
  
 
* '''Protein family:''' cytochrome P450 family (according to Swiss-Prot)
 
* '''Protein family:''' cytochrome P450 family (according to Swiss-Prot)
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* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
  
* '''Interactions:'''
+
* '''Interactions:''' [[YjiB]]-[[YkuN]] {{PubMed|20186410}}, [[YjiB]]-[[YkuP]] {{PubMed|20186410}}
  
 
* '''Localization:'''
 
* '''Localization:'''
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=References=
 
=References=
 
+
<pubmed>19591681 20186410  </pubmed>
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 10:22, 27 February 2010

  • Description: monooxygenase CYP109B1

Gene name yjiB
Synonyms
Essential no
Product monooxygenase CYP109B1
Function oxidation of fatty acids
MW, pI 44 kDa, 5.379
Gene length, protein length 1188 bp, 396 aa
Immediate neighbours yjiA, yjiC
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YjiB context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU12210

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: oxidation of saturated fatty acids (conversion up to 99%) and their methyl and ethyl esters (conversion up to 80%) at subterminal positions with a preference for the carbon atoms C11 and C12 counted from the carboxyl group PubMed
  • Protein family: cytochrome P450 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Marco Girhard, Tobias Klaus, Yogan Khatri, Rita Bernhardt, Vlada B Urlacher
Characterization of the versatile monooxygenase CYP109B1 from Bacillus subtilis.
Appl Microbiol Biotechnol: 2010, 87(2);595-607
[PubMed:20186410] [WorldCat.org] [DOI] (I p)

Marco Girhard, Kazuhiro Machida, Masashi Itoh, Rolf D Schmid, Akira Arisawa, Vlada B Urlacher
Regioselective biooxidation of (+)-valencene by recombinant E. coli expressing CYP109B1 from Bacillus subtilis in a two-liquid-phase system.
Microb Cell Fact: 2009, 8;36
[PubMed:19591681] [WorldCat.org] [DOI] (I e)