PbuE

• Description: putative hypoxanthine exporter
  
  

Gene name	pbuE
Synonyms	ydhL
Essential	no
Product	putative hypoxanthine exporter
Function	nucleotide metabolism
MW, pI	41 kDa, 8.884
Gene length, protein length	1164 bp, 388 aa
Immediate neighbours	ydhK, gmuB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU05800

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: View classification (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: Q797E3

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:
  • induced by hypoxanthine and guanine, PurR-independent PubMed
  • induced by adenine (A-box) PubMed

• Regulatory mechanism:
  • A-box: adenine-controlled riboswitch, transcription termination/ antitermination PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

J Kenneth Wickiser, Ming T Cheah, Ronald R Breaker, Donald M Crothers
The kinetics of ligand binding by an adenine-sensing riboswitch.
Biochemistry: 2005, 44(40);13404-14
[PubMed:16201765] [WorldCat.org] [DOI] (P p)

Per Nygaard, Hans H Saxild
The purine efflux pump PbuE in Bacillus subtilis modulates expression of the PurR and G-box (XptR) regulons by adjusting the purine base pool size.
J Bacteriol: 2005, 187(2);791-4
[PubMed:15629952] [WorldCat.org] [DOI] (P p)

Maumita Mandal, Ronald R Breaker
Adenine riboswitches and gene activation by disruption of a transcription terminator.
Nat Struct Mol Biol: 2004, 11(1);29-35
[PubMed:14718920] [WorldCat.org] [DOI] (P p)

Lars Engholm Johansen, Per Nygaard, Catharina Lassen, Yvonne Agersø, Hans H Saxild
Definition of a second Bacillus subtilis pur regulon comprising the pur and xpt-pbuX operons plus pbuG, nupG (yxjA), and pbuE (ydhL).
J Bacteriol: 2003, 185(17);5200-9
[PubMed:12923093] [WorldCat.org] [DOI] (P p)