DisA

• Description: DNA integrity scanning protein, delays sporulation in the case of chromosome damage, has diadenylate cyclase activity
  
  

Gene name	disA
Synonyms	yacK
Essential	no
Product	DNA integrity scanning protein, has diadenylate cyclase activity
Function	control of sporulation initiation
Metabolic function and regulation of this protein in SubtiPathways: Stress
MW, pI	40 kDa, 5.569
Gene length, protein length	1080 bp, 360 aa
Immediate neighbours	radA, yacL
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU00880

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: disA family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: RacA

• Localization: see a video showing the movement of DisA in the cell (in real time) PubMed

Database entries

• Structure: 3C23

• UniProt: P37573

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • ctsR-mcsA-mcsB-clpC-radA-disA PubMed
  • disA PubMed

• Sigma factor:
  • ctsR: SigA PubMed, SigB PubMed1 PubMed2
  • disA: SigM PubMed

• Regulation: induced by stress (SigB) PubMed, induced by heat (CtsR) PubMed

• Regulatory mechanism: CtsR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ute Römling
Great times for small molecules: c-di-AMP, a second messenger candidate in Bacteria and Archaea.
Sci Signal: 2008, 1(33);pe39
[PubMed:18714086] [WorldCat.org] [DOI] (I e)

Gregor Witte, Sophia Hartung, Katharina Büttner, Karl-Peter Hopfner
Structural biochemistry of a bacterial checkpoint protein reveals diadenylate cyclase activity regulated by DNA recombination intermediates.
Mol Cell: 2008, 30(2);167-78
[PubMed:18439896] [WorldCat.org] [DOI] (I p)

Adrian J Jervis, Penny D Thackray, Chris W Houston, Malcolm J Horsburgh, Anne Moir
SigM-responsive genes of Bacillus subtilis and their promoters.
J Bacteriol: 2007, 189(12);4534-8
[PubMed:17434969] [WorldCat.org] [DOI] (P p)

Michal Bejerano-Sagie, Yaara Oppenheimer-Shaanan, Idit Berlatzky, Alex Rouvinski, Mor Meyerovich, Sigal Ben-Yehuda
A checkpoint protein that scans the chromosome for damage at the start of sporulation in Bacillus subtilis.
Cell: 2006, 125(4);679-90
[PubMed:16713562] [WorldCat.org] [DOI] (P p)

Erik Boye
DisA, a busy bee that monitors chromosome integrity.
Cell: 2006, 125(4);641-3
[PubMed:16713555] [WorldCat.org] [DOI] (P p)

Sigal Ben-Yehuda, David Z Rudner, Richard Losick
RacA, a bacterial protein that anchors chromosomes to the cell poles.
Science: 2003, 299(5606);532-6
[PubMed:12493822] [WorldCat.org] [DOI] (I p)

A Petersohn, M Brigulla, S Haas, J D Hoheisel, U Völker, M Hecker
Global analysis of the general stress response of Bacillus subtilis.
J Bacteriol: 2001, 183(19);5617-31
[PubMed:11544224] [WorldCat.org] [DOI] (P p)

I Derré, G Rapoport, T Msadek
CtsR, a novel regulator of stress and heat shock response, controls clp and molecular chaperone gene expression in gram-positive bacteria.
Mol Microbiol: 1999, 31(1);117-31
[PubMed:9987115] [WorldCat.org] [DOI] (P p)

E Krüger, T Msadek, M Hecker
Alternate promoters direct stress-induced transcription of the Bacillus subtilis clpC operon.
Mol Microbiol: 1996, 20(4);713-23
[PubMed:8793870] [WorldCat.org] [DOI] (P p)