IspG

• Description: similar to peptidoglycan acetylation, 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate synthase
  
  

Gene name	ispG
Synonyms	yqfY
Essential	yes PubMed
Product	unknown
Function	unknown
MW, pI	40 kDa, 6.802
Gene length, protein length	1131 bp, 377 aa
Immediate neighbours	yqfZ, yqfX
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU25070

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H₂O + protein-disulfide = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + protein-dithiol (according to Swiss-Prot)

• Protein family: ispG family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: secreted (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P54482

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Mattijs K Julsing, Michael Rijpkema, Herman J Woerdenbag, Wim J Quax, Oliver Kayser
Functional analysis of genes involved in the biosynthesis of isoprene in Bacillus subtilis.
Appl Microbiol Biotechnol: 2007, 75(6);1377-84
[PubMed:17458547] [WorldCat.org] [DOI] (P p)