GcvPA

• Description: glycine decarboxylase (subunit 1)
  
  

Gene name	gcvPA
Synonyms	yqhJ, gcvP
Essential	no
Product	glycine decarboxylase (subunit 1)
Function	glycine utilization
MW, pI	49 kDa, 6.947
Gene length, protein length	1344 bp, 448 aa
Immediate neighbours	gcvPB, gcvT
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU24560

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Glycine + H-protein-lipoyllysine = H-protein-S-aminomethyldihydrolipoyllysine + CO₂ (according to Swiss-Prot)

• Protein family: N-terminal subunit subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P54376

• KEGG entry: [3]

• E.C. number: 1.4.4.2

Expression and regulation

• Operon: gcvT-gcvPA-gcvPB PubMed

• Sigma factor:

• Regulation: repressed by casamino acids PubMed , induced by glycine PubMed

• Regulatory mechanism: induction requires a co-operatively acting riboswitch PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Maumita Mandal, Mark Lee, Jeffrey E Barrick, Zasha Weinberg, Gail Mitchell Emilsson, Walter L Ruzzo, Ronald R Breaker
A glycine-dependent riboswitch that uses cooperative binding to control gene expression.
Science: 2004, 306(5694);275-9
[PubMed:15472076] [WorldCat.org] [DOI] (I p)

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)