DefB

• Description: major formylmethionine deformylase
  
  

Gene name	ykrB
Synonyms	defB
Essential	no
Product	formylmethionine deformylase
Function	translation
MW, pI	20 kDa, 5.456
Gene length, protein length	552 bp, 184 aa
Immediate neighbours	ykrA, ykyA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU14560

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Formyl-L-methionyl peptide + H₂O = formate + methionyl peptide (according to Swiss-Prot)

• Protein family: polypeptide deformylase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1LQY (complex with antibiotic actinonin, Geobacillus stearothermophilus)

• UniProt: Q45495

• KEGG entry: [2]

• E.C. number: 3.5.1.88

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Michael Haas, Dieter Beyer, Reinhold Gahlmann, Christoph Freiberg
YkrB is the main peptide deformylase in Bacillus subtilis, a eubacterium containing two functional peptide deformylases.
Microbiology (Reading): 2001, 147(Pt 7);1783-1791
[PubMed:11429456] [WorldCat.org] [DOI] (P p)