Difference between revisions of "DisA"

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* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=3C23 3C23]
 
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=3C23 3C23]
  
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P37573 P37573]
+
* '''UniProt:''' [http://www.uniprot.org/uniprot/P37573 P37573]
  
 
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU00880]
 
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU00880]

Revision as of 09:45, 20 July 2009

  • Description: DNA integrity scanning protein, delays sporulation in the case of chromosome damage, has diadenylate cyclase activity

Gene name disA
Synonyms yacK
Essential no
Product DNA integrity scanning protein, has diadenylate cyclase activity
Function control of sporulation initiation
Metabolic function and regulation of this protein in SubtiPathways:
Stress
MW, pI 40 kDa, 5.569
Gene length, protein length 1080 bp, 360 aa
Immediate neighbours radA, yacL
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YacK context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU00880

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: disA family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization: see a video showing the movement of DisA in the cell (in real time) PubMed

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

disA

disA: SigM PubMed

  • Regulatory mechanism: CtsR: transcription repression PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Ute Römling
Great times for small molecules: c-di-AMP, a second messenger candidate in Bacteria and Archaea.
Sci Signal: 2008, 1(33);pe39
[PubMed:18714086] [WorldCat.org] [DOI] (I e)

Gregor Witte, Sophia Hartung, Katharina Büttner, Karl-Peter Hopfner
Structural biochemistry of a bacterial checkpoint protein reveals diadenylate cyclase activity regulated by DNA recombination intermediates.
Mol Cell: 2008, 30(2);167-78
[PubMed:18439896] [WorldCat.org] [DOI] (I p)

Adrian J Jervis, Penny D Thackray, Chris W Houston, Malcolm J Horsburgh, Anne Moir
SigM-responsive genes of Bacillus subtilis and their promoters.
J Bacteriol: 2007, 189(12);4534-8
[PubMed:17434969] [WorldCat.org] [DOI] (P p)

Michal Bejerano-Sagie, Yaara Oppenheimer-Shaanan, Idit Berlatzky, Alex Rouvinski, Mor Meyerovich, Sigal Ben-Yehuda
A checkpoint protein that scans the chromosome for damage at the start of sporulation in Bacillus subtilis.
Cell: 2006, 125(4);679-90
[PubMed:16713562] [WorldCat.org] [DOI] (P p)

Erik Boye
DisA, a busy bee that monitors chromosome integrity.
Cell: 2006, 125(4);641-3
[PubMed:16713555] [WorldCat.org] [DOI] (P p)

Sigal Ben-Yehuda, David Z Rudner, Richard Losick
RacA, a bacterial protein that anchors chromosomes to the cell poles.
Science: 2003, 299(5606);532-6
[PubMed:12493822] [WorldCat.org] [DOI] (I p)

A Petersohn, M Brigulla, S Haas, J D Hoheisel, U Völker, M Hecker
Global analysis of the general stress response of Bacillus subtilis.
J Bacteriol: 2001, 183(19);5617-31
[PubMed:11544224] [WorldCat.org] [DOI] (P p)

I Derré, G Rapoport, T Msadek
CtsR, a novel regulator of stress and heat shock response, controls clp and molecular chaperone gene expression in gram-positive bacteria.
Mol Microbiol: 1999, 31(1);117-31
[PubMed:9987115] [WorldCat.org] [DOI] (P p)

E Krüger, T Msadek, M Hecker
Alternate promoters direct stress-induced transcription of the Bacillus subtilis clpC operon.
Mol Microbiol: 1996, 20(4);713-23
[PubMed:8793870] [WorldCat.org] [DOI] (P p)