Difference between revisions of "TrpS"
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* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1BM2 1BM2] (complex with tryptophan, Geobacillus stearothermophilus), [http://www.rcsb.org/pdb/explore.do?structureId=1MAW 1MAW] (complex with ATP, Geobacillus stearothermophilus) | * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1BM2 1BM2] (complex with tryptophan, Geobacillus stearothermophilus), [http://www.rcsb.org/pdb/explore.do?structureId=1MAW 1MAW] (complex with ATP, Geobacillus stearothermophilus) | ||
| − | * ''' | + | * '''UniProt:''' [http://www.uniprot.org/uniprot/P21656 P21656] |
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU11420] | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU11420] | ||
Revision as of 11:54, 20 July 2009
- Description: tryptophany-tRNA synthetase
| Gene name | trpS |
| Synonyms | |
| Essential | yes PubMed |
| Product | tryptophany-tRNA synthetase |
| Function | translation |
| Metabolic function and regulation of this protein in SubtiPathways: tRNA charging | |
| MW, pI | 37 kDa, 6.542 |
| Gene length, protein length | 990 bp, 330 aa |
| Immediate neighbours | yjbA, oppA |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU11420
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp) (according to Swiss-Prot)
- Protein family: class-I aminoacyl-tRNA synthetase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure: 1BM2 (complex with tryptophan, Geobacillus stearothermophilus), 1MAW (complex with ATP, Geobacillus stearothermophilus)
- UniProt: P21656
- KEGG entry: [3]
- E.C. number: 6.1.1.2
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532]
[WorldCat.org]
[DOI]
(I p)
A I Lee, J P Sarsero, C Yanofsky
A temperature-sensitive trpS mutation interferes with trp RNA-binding attenuation protein (TRAP) regulation of trp gene expression in Bacillus subtilis.
J Bacteriol: 1996, 178(22);6518-24
[PubMed:8932308]
[WorldCat.org]
[DOI]
(P p)
