Pcp

• Description: pyrrolidone-carboxylate peptidase
  
  

Gene name	pcp
Synonyms
Essential	no
Product	pyrrolidone-carboxylate peptidase
Function	removal of the N-terminal pyroglutamyl group from peptides
MW, pI	23 kDa, 6.418
Gene length, protein length	645 bp, 215 aa
Immediate neighbours	tatCD, ycbU
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU02650

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Release of an N-terminal pyroglutamyl group from a polypeptide, the second amino acid generally not being Pro (according to Swiss-Prot)

• Protein family: peptidase C15 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P28618

• KEGG entry: [2]

• E.C. number: 3.4.11.8

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

T Gonzalès, A Awadé, C Besson, J Robert-Baudouy
Purification and characterization of recombinant pyrrolidone carboxyl peptidase of Bacillus subtilis.
J Chromatogr: 1992, 584(1);101-7
[PubMed:1362573] [DOI] (P p)

A Awadé, P Cleuziat, T Gonzalès, J Robert-Baudouy
Characterization of the pcp gene encoding the pyrrolidone carboxyl peptidase of Bacillus subtilis.
FEBS Lett: 1992, 305(1);67-73
[PubMed:1353026] [WorldCat.org] [DOI] (P p)